CRISPR-Cas Functional Genetic Screening: Traditional Review

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dc.authorscopusid57219431760
dc.authorscopusid56508201600
dc.authorscopusid57222185626
dc.authorscopusid24830456300
dc.authorscopusid12545373100
dc.contributor.authorGüler Kara H.
dc.contributor.authorKosova B.
dc.contributor.authorDoğan E.
dc.contributor.authorBozok Çetintaş V.
dc.contributor.authorŞentürk Ş.
dc.date.accessioned2023-01-12T20:26:32Z
dc.date.available2023-01-12T20:26:32Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractThe clustered regularly interspaced palindromic repeats (CRISPR)-associated Cas system, derived from the defence mechanism components of prokaryotes, is frequently used as a target specific gene editing tool in in vitro and in vivo studies. Uses of the CRISPR-Cas system include gene deletion, gene insertion, base editing, gene expression editing, genomic locus imaging, epigenetic editing, and diagnostic analysis. Due to the strengths of the system such as flexibility, versatility and easy application, its usage areas have gradually increased. In recent years, CRISPR-Cas9 based functional genetic screen studies have been carried out to understand the roles of genes in the biological processes and cellular functions. CRISPR screens are used to identify genetic factors responsible for disease development and determine their functions, which are necessary for the survival of cells due to disease, especially cancer. CRISPR-Cas9 screens consisting of desing and amplification of the selected gRNA library, cloning in the relevant plasmid, packaging into lentiviruses and lentiviral transduction into cells, phenotypic selection, next generation DNA sequence analysis and bioinformatics analysis, has allowed functional genetic studies that can be performed with high-throughput and high-efficiency. Screening studies can be carried out for different purposes such as investigating pathogen-host interactions, revealing the molecular mechanisms of diseases, identifying novel therapeutic targets and revealing factors that play a key role in treatment resistance. In this review, after a short summary of different CRISPR-Cas systems the basic principles of genetic screening studies, their application and areas of usage will be discussed with selected examples from the current literature. © 2022 by Türkiye Klinikleri.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK: 119Z280/1001en_US
dc.description.sponsorshipFinansal Kaynak Bu çalışma, TÜBİTAK Kurulu tarafından onaylanmış (Proje no: 119Z280/1001) ve TÜBİTAK araştırma fonunca desteklenmiştir.en_US
dc.identifier.doi10.5336/medsci.2022-88507
dc.identifier.endpage322en_US
dc.identifier.issn13000292
dc.identifier.issn1300-0292en_US
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85143511577en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage311en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2022-88507
dc.identifier.urihttps://hdl.handle.net/11454/80043
dc.identifier.volume42en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherTurkiye Kliniklerien_US
dc.relation.ispartofTurkiye Klinikleri Journal of Medical Sciencesen_US
dc.relation.publicationcategoryDiğeren_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCRISPR screenen_US
dc.subjectCRISPR-Casen_US
dc.subjectfunctional genetic screenen_US
dc.subjectgRNA libraryen_US
dc.subjectbioinformaticsen_US
dc.subjectcell survivalen_US
dc.subjectclustered regularly interspaced short palindromic repeaten_US
dc.subjectCRISPR Cas systemen_US
dc.subjectdisease courseen_US
dc.subjectDNA sequenceen_US
dc.subjectepigenetic modificationen_US
dc.subjectgene amplificationen_US
dc.subjectgene editingen_US
dc.subjectgene expressionen_US
dc.subjectgene insertionen_US
dc.subjectgene libraryen_US
dc.subjectgene locusen_US
dc.subjectgenetic screeningen_US
dc.subjectgenetic transductionen_US
dc.subjectheredityen_US
dc.subjecthigh throughput sequencingen_US
dc.subjectin vitro studyen_US
dc.subjectin vivo studyen_US
dc.subjectLentivirusen_US
dc.subjectnonhumanen_US
dc.subjectphenotypic variationen_US
dc.subjectplasmiden_US
dc.subjectprokaryoteen_US
dc.subjectReviewen_US
dc.titleCRISPR-Cas Functional Genetic Screening: Traditional Reviewen_US
dc.title.alternativeCRISPR-Cas Fonksiyonel Genetik Tarama: Geleneksel Derlemeen_US
dc.typeReviewen_US

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