Characterization, immobilization, and activity enhancement of cellulase treated with supercritical CO2

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dc.contributor.authorSenyay-Oncel, Deniz
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T22:59:07Z
dc.date.available2019-10-27T22:59:07Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractEnzyme activity enhancement represents a great opportunity for biotechnological production, while recovery of most enzymes from media, possible loss of catalytic activity in reactions, and denaturation occur. In this study, the activity and stability enhancement, kinetic parameters, and thermal inactivation for cellulose hydrolysis of supercritical carbon dioxide (SC-CO2)-treated and untreated cellulase from Trichoderma longibrachiatum as a model enzyme are presented, and the activity enhancement capability of SC-CO2 for cellulase after consecutive enzymatic reactions is discussed. The pH and temperature stability were pH 5 and 50 A degrees C for the enzymatic reaction, whereas the kinetic parameter values, V (max) and K (m), were calculated using the Michaelis-Menten model. The optimal operational parameters were determined to be 54 A degrees C, 180 bar, and 10 g/min CO2 flow rate for 120 min, yielding 48.3 % higher activity (9.27 mu mol/ml/min) than for untreated enzyme. In addition, SC-CO2-treated cellulase with the highest activity was immobilized on NaY zeolite, and consecutive reactions were carried out. The presented results suggest that enzymes as catalysts in biochemical applications can be improved by using supercritical fluids as potential media.en_US
dc.identifier.doi10.1007/s10570-015-0780-2
dc.identifier.endpage3631en_US
dc.identifier.issn0969-0239
dc.identifier.issn1572-882X
dc.identifier.issn0969-0239en_US
dc.identifier.issn1572-882Xen_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage3619en_US
dc.identifier.urihttps://doi.org/10.1007/s10570-015-0780-2
dc.identifier.urihttps://hdl.handle.net/11454/51726
dc.identifier.volume22en_US
dc.identifier.wosWOS:000364513800010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCelluloseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSupercritical CO2en_US
dc.subjectCellulaseen_US
dc.subjectEnzyme activityen_US
dc.subjectKinetic parametersen_US
dc.titleCharacterization, immobilization, and activity enhancement of cellulase treated with supercritical CO2en_US
dc.typeArticleen_US

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