Electrochemical genoassay design for allele-specific detection of toll-like receptor-2 gene polymorphism

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dc.contributor.authorKara, Pinar
dc.contributor.authorCavdar, Seda
dc.contributor.authorBerdeli, Afig
dc.contributor.authorOzsoz, Mehmet
dc.date.accessioned2019-10-27T19:32:39Z
dc.date.available2019-10-27T19:32:39Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractAn allele-specific voltammetric genoassay for the detection of allele-specific toll-like receptor-2 gene arg753gln polymorphism (TLR-2) from polymerase chain reaction (PCR) amplified real samples was described in this study. Meldola blue (MDB), an intercalator molecule, was used as hybridization label. The wild-type and mutant type oligonucleotide probes were immobilized onto disposable graphite electrode surfaces by covalent attachment method. The extent of hybridization between probe and target sequences was determined by using differential pulse voltammetry (DPV). As a result of the interaction between MDB and DNA at electrode surface, the MDB signal observed from probe sequence before hybridization and after hybridization with MM sequence is lower than that observed after hybridization with complementary sequence. The differences between the MDB reduction peaks obtained from probe modified, hybrid modified and MM modified electrode were used to detect TLR-2 from PCR amplified real samples. The discrimination of homozygous and heterozygous alleles was also established by comparing the peak currents of MDB reduction signals. Numerous factors affecting the target hybridization and indicator binding reactions are optimized to maximize the sensitivity.en_US
dc.identifier.doi10.1002/elan.200703944
dc.identifier.endpage1882en_US
dc.identifier.issn1040-0397
dc.identifier.issn1040-0397en_US
dc.identifier.issue18en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1875en_US
dc.identifier.urihttps://doi.org/10.1002/elan.200703944
dc.identifier.urihttps://hdl.handle.net/11454/39326
dc.identifier.volume19en_US
dc.identifier.wosWOS:000249630900003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectelectrochemical genosensoren_US
dc.subjectintercalationen_US
dc.subjecttoll-like receptorsen_US
dc.subjectpolymorphismen_US
dc.subjectpolymerase chain reactionen_US
dc.titleElectrochemical genoassay design for allele-specific detection of toll-like receptor-2 gene polymorphismen_US
dc.typeArticleen_US

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