Granulocyte Colony-Stimulating Factor Prevents Ischemia/Reperfusion-Induced Ovarian Injury in Rats: Evaluation of Histological and Biochemical Parameters

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dc.contributor.authorHortu I.
dc.contributor.authorOzceltik G.
dc.contributor.authorSahin C.
dc.contributor.authorAkman L.
dc.contributor.authorYildirim N.
dc.contributor.authorErbas O.
dc.date.accessioned2019-10-26T21:15:32Z
dc.date.available2019-10-26T21:15:32Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractGranulocyte colony-stimulating factor (G-CSF) is a glycoprotein commonly used in the field of medicine to treat neutropenia. Granulocyte colony-stimulating factor has also crucial roles in ameliorating the ischemia/reperfusion (I/R) injury in particular tissues. In this study, we aimed to investigate the protective effect of G-CSF on ovarian damage in experimental ovarian I/R injury. Thirty adult female rats were used. Rats were separated randomly into 5 groups; Group 1: sham group (abdominal wall was opened and closed surgically), Group 2: torsion group with 3-hour ischemia using vascular clips. Group 3: torsion + G-CSF group with 3-hour ischemia 30 minutes after the administration intraperitoneal (i.p.) of 100 µg/kg of G-CSF. Group 4: torsion-detorsion group with 3 hour ischemia and 3 hour reperfusion. Group 5: torsion-detorsion + G-CSF group with 3 hour ischemia followed by 100 µg/kg of G-CSF i.p. administration 30 minutes prior to 3 hour of detorsion/reperfusion. Ovarian tissue damage was scored on histopathology. Ovarian tissue malondialdehyde (MDA) was measured biochemically. In comparison with the sham group, both the torsion and torsion-detorsion groups had significantly higher scores for follicular degeneration, vascular congestion, edema, hemorrhage, and leukocyte infiltration (P <.05). When compared group torsion-detorsion + G-CSF to group torsion-detorsion, parameters aforementioned significantly decreased in group torsion-detorsion + G-CSF (P <.05). Granulocyte colony-stimulating factor has also decreased MDA levels notably both in the torsion + G-CSF and torsion-detorsion + G-CSF groups (P <.05, P <.01). Our experimental study suggests that G-CSF can be a novel agent for the treatment of ovarian I/R injury. © The Author(s) 2018.en_US
dc.identifier.doi10.1177/1933719118816839
dc.identifier.issn1933-7191
dc.identifier.issn1933-7191en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1177/1933719118816839
dc.identifier.urihttps://hdl.handle.net/11454/16040
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSAGE Publications Inc.en_US
dc.relation.ispartofReproductive Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectgranulocyte colony-stimulating factoren_US
dc.subjectischemia-reperfusion injuryen_US
dc.subjectovarian torsionen_US
dc.subjectrat modelen_US
dc.titleGranulocyte Colony-Stimulating Factor Prevents Ischemia/Reperfusion-Induced Ovarian Injury in Rats: Evaluation of Histological and Biochemical Parametersen_US
dc.typeArticleen_US

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