Composition of Carotenoids in Scenedesmus protuberans: Application of Chromatographic and Spectroscopic Methods
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer New York LLC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study aimed to identify and determine the carotenoids from green microalga, Scenedesmus protuberans using analytical techniques. Identification of carotenoids was realized by comparing their absorption and mass spectral data with those of reference standards available and reported values. Chromatographic data were then combined with the spectroscopic information. The separation of carotenoids was achieved by C<inf>30</inf> column and high-performance liquid chromatography-diode array detection was used for their determination. In the present work, the carotenoid content of S. protuberans was found to be 1.45 mg/g of violaxanthin, 2.47 mg/g of all-trans-lutein, 0.15 mg/g of all-trans-?-carotene, 0.55 mg/g of all-trans-ß-carotene, and 0.20 mg/g of 9 or 9'-cis-ß-carotene. Due to lack of their standards, the amount of all-trans-loroxanthin and cis-isomers of other carotenoids could not be quantified. In order to validate the method, Certified Reference Material (BCR 485-Mixed vegetables) was used. In conclusion, this study can serve as a reference for the analysis of carotenoids in other microalgae. © 2015, Springer Science+Business Media New York.
Açıklama
Anahtar Kelimeler
Carotenoids, Chromatography and spectroscopy, Extraction, Method validation, Scenedesmus protuberans
Kaynak
Food Analytical Methods
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
8
Sayı
8
