Catalase-conjugated surfaces: H2O2 detection based on quenching of tryptophan fluorescence on conducting polymers

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dc.contributor.authorGelen, Sultan Sacide
dc.contributor.authorMunkhbat, Tuvshinjargal
dc.contributor.authorRexhepi, Zuale
dc.contributor.authorKirbay, Fatma Ozturk
dc.contributor.authorAzak, Hacer
dc.contributor.authorDemirkol, Dilek Odaci
dc.date.accessioned2021-05-03T20:33:18Z
dc.date.available2021-05-03T20:33:18Z
dc.date.issued2021
dc.departmentEge Üniversitesien_US
dc.description.abstractConducting polymers are promising materials used to prepare electrochemical and optical detection platforms for bioanalytical systems. After conjugation of biomolecules onto electmpolymerized monomers for preparation of multifunctional surfaces, they can be used to easily monitor small molecules, macromolecules, and cells. in this study, multifunctional monomer with indole (necessary for fluorescence detection of H2O2) and carboxyl functional groups (necessary for covalent immobilization of biological material) was synthesized, electropolymerized, modified with biomolecules (catalase) and then applied for the selective detection of target analyte (H2O2). Tryptophan sequestered dithione [3,2-b:2',3'-d] pyrmle (DTP-Trp) was synthesized and electropolymerized on indium tin oxide (ITO) coated polyethylene terephthalate (PET) substrates, which is a transparent support. Afterward, catalase (CAT) was immobilized on the Poly(DTP-Trp) using a zero-length crosslinker, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), via covalent bonds between carboxyl groups of CAT and amino groups of Poly(DTP-Trp). Surface characterization of Poly(DTP-Trp)/CAT was done by scanning electron microscopy (SEM) and energy dispersive x-ray analysis (EDX). The presence of Poly(DTP-Trp)/CAT on ITO-PET surfaces were also confirmed by electrochemical methods to show the success of support modification. Determination of H2O2 was investigated using fluorescence spectrometry based on quenching mechanism of H2O2 on the fluorescence of tryptophan. After optimization of working conditions of Poly(DTPTrp)/CAT, H2O2 detection in synthetic samples was done without any interference of the matrices.en_US
dc.identifier.doi10.1016/j.eurpolymj.2020.110130
dc.identifier.issn0014-3057
dc.identifier.issn1873-1945
dc.identifier.urihttps://doi.org/10.1016/j.eurpolymj.2020.110130
dc.identifier.urihttps://hdl.handle.net/11454/69950
dc.identifier.volume142en_US
dc.identifier.wosWOS:000603476700017en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofEuropean Polymer Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFluorescence sensingen_US
dc.subjectQuenching of fluorescenceen_US
dc.subjectConducting polymeren_US
dc.subjectBiofunctionalized surfaceen_US
dc.subjectCatalaseen_US
dc.subjectPeroxide detectionen_US
dc.titleCatalase-conjugated surfaces: H2O2 detection based on quenching of tryptophan fluorescence on conducting polymersen_US
dc.typeArticleen_US

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