Development of an Integrated Optical Sensor for Determination of ?-Hydroxybutyrate Within the Microplatform

Basit Öğe Kaydı
dc.contributor.authorDevamoglu U.
dc.contributor.authorDuman I.
dc.contributor.authorSaygili E.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2021-05-03T20:57:07Z
dc.date.available2021-05-03T20:57:07Z
dc.date.issued2021
dc.description.abstractKetone bodies (acetoacetate, beta-hydroxybutyrate (?HB), acetone) are generated as a result of fatty acid oxidation in the liver and exist at low concentrations in urine and blood. Elevated concentrations can indicate health problems such as diabetes, childhood hypoglycemia, alcohol, or salicylate poisoning. Development of portable and cost-effective bedside point-of-care (POC) tests to detect such compounds can help to reduce the risk of disease progression. In this study, ?HB was chosen as a model molecule for developing an optical sensor–integrated microplatform. Prior to sensor optimization, ?HB levels were measured at a concentration range of 0.02 and 0.1 mM spectrophotometrically, which is far below the reported elevated ranges of 1–2 mM and resulting absorbance changes were converted into an Arduino microcontroller code for the correlation. Measurements performed with the designed integrated microplatform were found significant. Integrated microplatform was verified with the benchtop spectrophotometer. Measurements between 0.02 and 0.1 mM substrate concentration were found highly sensitive with “y = 0.7347x + 0.00184” with R2 value of 0.9796, and the limit of detection was determined as 0.02 mM. Based on these results, the proposed system will allow on-site and early intervention. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.description.sponsorship1919B011800241 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKen_US
dc.description.sponsorshipThe study was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) 2209/A University Students Research Project Support Program (1919B011800241).en_US
dc.description.sponsorshipThe authors are grateful to Armagan Ergun for his contributions to PMMA mold production. E.S gratefully acknowledges the TUBITAK 2211-A National Graduate Scholarship Program.en_US
dc.identifier.doi10.1007/s12010-021-03563-3
dc.identifier.issn0273-2289
dc.identifier.issn0273-2289en_US
dc.identifier.pmid33834362en_US
dc.identifier.scopus2-s2.0-85104077456en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-021-03563-3
dc.identifier.urihttps://hdl.handle.net/11454/71499
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectKetone bodiesen_US
dc.subjectMicroplatformen_US
dc.subjectOptical sensoren_US
dc.subjectPoint-of-care testsen_US
dc.titleDevelopment of an Integrated Optical Sensor for Determination of ?-Hydroxybutyrate Within the Microplatformen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu