Development of Ketoprofen Solid Dispersions and Evaluation of Their Protective Effects against DNA Damage and Cytotoxicity Potential

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dc.contributor.authorRencber, Seda
dc.contributor.authorOzyazici, Mine
dc.contributor.authorTurunc, Ezgi
dc.contributor.authorTascioglu, Alev
dc.contributor.authorGurer Orhan, Hande
dc.contributor.authorYalcin, Ayfer
dc.date.accessioned2019-10-27T11:18:53Z
dc.date.available2019-10-27T11:18:53Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractThe purpose of this study was to prepare solid dispersion formulations (SDs) of ketoprofen (KP) in order to increase solubility and dissolution rate. This approach is important because of the potential usage of these formulations for neurodegenerative diseases such as Alzheimer's. SDs of KP in different ratios as drug-polymer system were prepared using melting method and ideal formulation was selected by in vitro and in situ studies. In situ effects of these developed formulations on DNA damage was determined in rat brain cortex homogenates. Our results showed that SDs helped to improve the dissolution rates of KP. These formulations decreased the levels of DNA damage in rat brain cortex when compared to controls. The selected B3 formulation would have a potential protective effect on neurodegenerative processes including inflammation. In addition, cytotoxic potential and long term effect of B3 formulation on cell survival were evaluated via MTT and colony formation assays, respectively. Tested formulation had no cytotoxic effect and did not decrease the cell survival at its pharmacologically relevant and higher concentrations. Overall, newly developed SD formulation of KP is suggested to be a promising candidate for long term use in neurodegenerative diseases because of its improved solubility and dissolution rates, increased beneficial activity against DNA damage and its short and long term safety in cells.en_US
dc.description.sponsorshipEge UniversityEge University [08/ECZ/002]en_US
dc.description.sponsorshipThis study was supported by a research grant from Ege University (Project number: 08/ECZ/002).en_US
dc.identifier.endpage2475en_US
dc.identifier.issn0326-2383
dc.identifier.issn0326-2383en_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage2464en_US
dc.identifier.urihttps://hdl.handle.net/11454/32642
dc.identifier.volume36en_US
dc.identifier.wosWOS:000425930400020en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherColegio Farmaceuticos Provincia De Buenos Airesen_US
dc.relation.ispartofLatin American Journal of Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlzheimer's diseaseen_US
dc.subjectcytotoxicityen_US
dc.subjectDNA damageen_US
dc.subjectketoprofenen_US
dc.subjectpoorly soluble drugen_US
dc.subjectsolid dispersionen_US
dc.titleDevelopment of Ketoprofen Solid Dispersions and Evaluation of Their Protective Effects against DNA Damage and Cytotoxicity Potentialen_US
dc.typeArticleen_US

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