Polyclonal Antibody Production Against Hapten-Structured KDN Molecule by Using Different Adjuvants Alternative to Freund's Adjuvant

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dc.contributor.authorIz, Sultan Gulce
dc.contributor.authorMetiner, Pelin Saglam
dc.contributor.authorKimiz, Ilgin
dc.contributor.authorKayali, Caglar
dc.contributor.authorGurhan, Saime Ismet Deliloglu
dc.date.accessioned2019-10-27T10:05:11Z
dc.date.available2019-10-27T10:05:11Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid), a member of the sialic acid family, is a hapten-structured low-molecular-weight monosaccharide on the cell membrane, which cannot induce immune responses without a carrier protein. Since it is over-expressed on the cancerous cells' membrane, it is thought to be a great target molecule for anti-cancer treatments. The aim of this study is to obtain high titersof the anti-KDN polyclonal antibody response without using any carrier protein against the hapten-structured KDN molecule alternative to Freund's adjuvants. Methods: Montanide (TM) ISA 61 VG, a water-in-oil adjuvant; ISA 201 VG, a water-in-oil-in-water emulsion adjuvant; and IMS 1313 VG NPR, an aqueous-dispersion-based nanoparticle (50-200 nm) microemulsion adjuvant; and Freund's adjuvant were used as anti-KDN antibody response stimulators. FourBALB/c mice were used for each adjuvant group, and immunization was performed at eight different time points. Anti-KDN antibody levels induced after each immunization with different adjuvants were detected with indirect enzyme-linked immunosorbent assay. Results: The adjuvant efficiency of Montanide (TM) ISA 61 VG water in oil adjuvant was 1.4 times higher than in Freund's adjuvant (p<0.0001), with a maximum anti-KDN level on Day 83. Conclusion: It's shown that without any carrier protein conjugation molecules such as hapten-structured KDN, higher amount anti-KDN antibody titres could be obtained by using a more safe and effective Montanide (TM) ISA 61 VG water-in-oil adjuvant as an alternative to Freund's adjuvants. In this regard, it may be possible to produce high-antibody titers without using any carrier molecule, especially when commercial large scale monoclonal antibodies are desired to be produced against haptens as therapeutic approaches.en_US
dc.description.sponsorshipTUBITAK-2209-B project [1139B411502819]en_US
dc.description.sponsorshipThis study was supported as part of TUBITAK-2209-B project which was carried out under the supervision of Assist Prof Sultan Gulce Iz. (Project Number: 1139B411502819).en_US
dc.identifier.doi10.5152/EurJTher.2018.400
dc.identifier.endpage111en_US
dc.identifier.issn2564-7784
dc.identifier.issn2564-7040
dc.identifier.issn2564-7784en_US
dc.identifier.issn2564-7040en_US
dc.identifier.issue2en_US
dc.identifier.startpage106en_US
dc.identifier.urihttps://doi.org/10.5152/EurJTher.2018.400
dc.identifier.urihttps://hdl.handle.net/11454/30327
dc.identifier.volume24en_US
dc.identifier.wosWOS:000439206700008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAves Press Ltden_US
dc.relation.ispartofEuropean Journal of Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectKDN (2-keto-3-deoxy-D-glycero-D-galacto-nonionic acid)en_US
dc.subjectpolyclonal antibodyen_US
dc.subjectELISAen_US
dc.titlePolyclonal Antibody Production Against Hapten-Structured KDN Molecule by Using Different Adjuvants Alternative to Freund's Adjuvanten_US
dc.typeArticleen_US

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