Carbapenemase investigation with rapid phenotypic test (RESIST-4 O.K.N.V) and comparison with PCR in carbapenem-resistant Enterobacterales strains

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dc.authorscopusid57194681921
dc.authorscopusid6507459615
dc.authorscopusid57196047480
dc.authorscopusid57219153428
dc.authorscopusid6508260544
dc.authorwosidPolat, Furkan/HGB-1047-2022
dc.contributor.authorYasar-Duman, Melike
dc.contributor.authorCilli, Feriha
dc.contributor.authorTekintas, Yamac
dc.contributor.authorPolat, Furkan
dc.contributor.authorHosgor-Limoncu, Mine
dc.date.accessioned2023-01-12T20:19:30Z
dc.date.available2023-01-12T20:19:30Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractBackground and Objectives: RESIST-4 O.K.N.V. assay is a lateral immunochromatocraphic test for the identification of oxacillinase (OXA)-48-like, Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-beta-lactamase (NDM), and Verona integron-encoded metallo-beta-lactamase (VIM) producing strains. It was aimed to evaluate the performance of the RESIST-4 O.K.N.V. test and to compare it with the reference method polymerase chain reaction (PCR). Also, the objective was to determine the distribution of carbapenemase types of CRE strains isolated in our hospital. Materials and Methods: Between January 2016-October 2019, 187 strains isolated from clinical samples were included in this study. Bacterial identification was done using MALDI-TOF MS. Antibiotic susceptibility tests were studied with the VITEK-2 automated system. Meropenem minimum inhibitory concentrations (MICs) were determined by the gradient test. All strains were studied with the RESIST-4 O.K.N.V. test and then the strains were selected for the PCR test. bla(OXA-48), bla(NDM), bla(KPC), and bla(VIM) were investigated with PCR. K. pneumoniae NCTC (R) 13438 (KWIKSTIKTM, Microbiologics (R),USA) was used as the positive control, E. coli ATTC (R) 25922 TM (Microbiologics (R),USA) and three carbapenem-sensitive clinical isolates were also used as the negative control. Results: Meropenem MIC50 and MIC90 values were determined to be >32 mg/ L. With PCR bla(OXA-48), bla(NDM), bla(KPC) and bla(VIM) were detected in 79, 63, 20, and 4 strains, respectively. bla(OXA-48) and bla(NDM) were found together in 51 of the isolates. bla(OXA-48), bla(NDM), bla(KPC), and bla(VIM) were not detected in two strains with carbapenem resistance in susceptibility tests. The sensitivity of the immunochromatographic test was 100% for OXA-48, KPC, and VIM but 84.1% for NDM. Specificity was determined as 100% for OXA-48, NDM, KPC, and VIM. Conclusion: RESIST-4 O.K.N.V. test showed high sensitivity and specificity in detecting OXA-48, KPC, NDM, and VIM type carbapenemases. However, it should be kept in mind that there may be false-negative results related to NDM.en_US
dc.identifier.endpage333en_US
dc.identifier.issn2008-3289
dc.identifier.issn2008-4447
dc.identifier.issn2008-3289en_US
dc.identifier.issn2008-4447en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85132244221en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage328en_US
dc.identifier.urihttps://hdl.handle.net/11454/79144
dc.identifier.volume14en_US
dc.identifier.wosWOS:000830512000008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Medical Sciences-Danishgah-I Ulum-I Pizishki-I Tihranen_US
dc.relation.ispartofIranian Journal Of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbapenemasesen_US
dc.subjectImmunochromatographic testen_US
dc.subjectOxacillinase-48en_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectReal-Time Pcren_US
dc.subjectNdm-1en_US
dc.subjectVimen_US
dc.subjectKpcen_US
dc.titleCarbapenemase investigation with rapid phenotypic test (RESIST-4 O.K.N.V) and comparison with PCR in carbapenem-resistant Enterobacterales strainsen_US
dc.typeArticleen_US

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