Isolation and Immobilization of His-Tagged Alcohol Dehydrogenase on Magnetic Nanoparticles in One Step: Application as Biosensor Platform

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dc.contributor.authorGuldu, Ozge Kozgus
dc.contributor.authorEce, Selin
dc.contributor.authorEvran, Serap
dc.contributor.authorMedine, E. Ilker
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorUnak, Perihan
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T22:06:25Z
dc.date.available2019-10-27T22:06:25Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractHis-tagged Alcohol dehydrogenase was produced as a recombinant protein in E. coli. Afterwards, isolation and immobilization of the enzyme was carried in one-step via copper modified magnetic nanoparticles (MNPs) by the effect of interactions between Cu and histidine. The resulting enzyme bound MNPs was then attached to the surface of carbon paste electrode by the magnetic force and used as an electrochemical biosensor for the alcohol sensing applications.en_US
dc.description.sponsorshipEge UniversityEge University [2010-ILAM-003]en_US
dc.description.sponsorshipThe authors thank the Ege University Research Funds (Project number: 2010-ILAM-003).en_US
dc.identifier.doi10.1080/10601325.2014.936236
dc.identifier.endpage705en_US
dc.identifier.issn1060-1325
dc.identifier.issn1520-5738
dc.identifier.issn1060-1325en_US
dc.identifier.issn1520-5738en_US
dc.identifier.issue9en_US
dc.identifier.startpage699en_US
dc.identifier.urihttps://doi.org/10.1080/10601325.2014.936236
dc.identifier.urihttps://hdl.handle.net/11454/48798
dc.identifier.volume51en_US
dc.identifier.wosWOS:000340145100003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal of Macromolecular Science Part A-Pure and Applied Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHis-tagged alcohol dehydrogenaseen_US
dc.subjectmagnetic nanoparticlesen_US
dc.subjectalcohol biosensingen_US
dc.titleIsolation and Immobilization of His-Tagged Alcohol Dehydrogenase on Magnetic Nanoparticles in One Step: Application as Biosensor Platformen_US
dc.typeArticleen_US

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