Screening and characterization of thermostable fibrinolytic enzyme from Bacillus amyloliquefaciens EGE-B-2d.1

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dc.contributor.authorSlem, Birkan
dc.contributor.authorGezgin, Yuksel
dc.contributor.authorEltem, Rengin
dc.date.accessioned2019-10-27T23:09:38Z
dc.date.available2019-10-27T23:09:38Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: To screen fibrinolytic enzyme-producing Bacillus isolates (n=210) and to characterize of thermostable fibrinolytic enzyme from Bacillus amyloliquefaciens EGE-B-2d.1 that had the highest level of fibrinolytic activity together with the highest thermostability. Methods: Firstly, a total of 210 isolates were screened for their fibrinolytic enzyme production. The potent fibrinolytic enzyme producing isolates were evaluated for the thermostability of their fibrinolytic enzymes and one isolate showing prominent fibrinolytic activity was identified as molecular. Fermentation process was carried out on the isolate that had both the highest level of fibrinolytic activity and enzyme thermostability. The thermostable fibrinolytic enzyme from this isolate was then purified and characterized. Results: The fibrinolytic enzyme activities of 21 Bacillus sp. isolates in Nutrient Yeast Salt Medium were found to be in the range of 0.176-1.734 U/ml. The fibrinolytic activity of the enzyme purified from the culture supernatant of Bacillus amyloliquefaciens EGE-B-2d.1 was relatively stable at pH 7.0-11.0 for 24 h and also showed stability at a temperature of 60 degrees C for 60 min. The enzyme degraded the fibrin clots by direct fibrinolysis. The specific activity and the molecular weight of the purified enzyme were estimated to be 44.46 units/mg protein and 30 kD respectively. Conclusion: The thermostable fibrinolytic enzyme from Bacillus amyloliquefaciens EGE-B-2d.1 was purified and characterized. This enzyme might also be used as a natural agent for oral fibrinolytic therapy or thrombosis prevention.en_US
dc.identifier.doi10.1515/tjb-2016-0027
dc.identifier.endpage176en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issn0250-4685en_US
dc.identifier.issn1303-829Xen_US
dc.identifier.issue3en_US
dc.identifier.startpage167en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2016-0027
dc.identifier.urihttps://hdl.handle.net/11454/52706
dc.identifier.volume41en_US
dc.identifier.wosWOS:000377541000006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFibrinolytic enzymeen_US
dc.subjectBacillus amyloliquefaciensen_US
dc.subjectproductionen_US
dc.subjectpurificationen_US
dc.subjectplasminogen activatoren_US
dc.subjectthrombolytic agenten_US
dc.titleScreening and characterization of thermostable fibrinolytic enzyme from Bacillus amyloliquefaciens EGE-B-2d.1en_US
dc.typeArticleen_US

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