Optimization of pullulan production from hydrolysed potato starch waste by response surface methodology

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dc.contributor.authorGoksungur, Yekta
dc.contributor.authorUzunogullari, Purlen
dc.contributor.authorDagbagli, Seval
dc.date.accessioned2019-10-27T21:25:14Z
dc.date.available2019-10-27T21:25:14Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractThe production of pullulan from hydrolysed potato starch waste by Aureobasidium pullulans P56 was investigated. The liquefaction of potato starch was done by Ca-alginate immobilized amyloglucosidase and pullulanase enzymes in a packed bed bioreactor. Various organic nitrogen sources were tested and none of the nitrogen sources gave pullulan concentrations as high as that obtained with yeast extract. Response surface methodology was used to investigate the effects of three factors (incubation time, initial substrate concentration and initial pH) on the concentration of pullulan in batch cultures of A. pullulans. No previous work has used statistical analysis on the optimization of process parameters in pullulan production from hydrolysed potato starch waste. Maximum pullulan concentration of 19.2 g/l was obtained at the optimum levels of process variables (incubation time 111.8 h, initial substrate concentration 79.4 g/l, initial pH 7.26). The optimization led to a 20% increase in pullulan concentration. (C) 2010 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipEge UniversityEge University [2009 MUH 035]en_US
dc.description.sponsorshipThis research was supported by Ege University Scientific Research Project Fund through Project No: 2009 MUH 035. We also thank Mrs. Huriye Goksungur for checking the language of the manuscript.en_US
dc.identifier.doi10.1016/j.carbpol.2010.09.047
dc.identifier.endpage1337en_US
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.issn0144-8617en_US
dc.identifier.issn1879-1344en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1330en_US
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2010.09.047
dc.identifier.urihttps://hdl.handle.net/11454/44785
dc.identifier.volume83en_US
dc.identifier.wosWOS:000286965300043en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCarbohydrate Polymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPullulanen_US
dc.subjectPolysaccharideen_US
dc.subjectCalcium alginateen_US
dc.subjectImmobilizationen_US
dc.subjectA. pullulansen_US
dc.subjectResponse surface methodologyen_US
dc.subjectAmyloglucosidaseen_US
dc.subjectPullulanaseen_US
dc.titleOptimization of pullulan production from hydrolysed potato starch waste by response surface methodologyen_US
dc.typeArticleen_US

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