Antioxidant, cytotoxic and iNOS activity of Liquidambar orientalis Mill. resin extracts

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dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorKaris, Mert
dc.contributor.authorKarakaya, Leyla
dc.contributor.authorAkgul, Yurdanur
dc.date.accessioned2019-10-27T23:09:48Z
dc.date.available2019-10-27T23:09:48Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: The aim of this study is to investigate the in vitro cytotoxicity and iNOS (inducible nitric oxide synthase) inhibitory, and antioxidant activity in order to assess the traditional usage of Liquidambar orientalis Mill resin extract. Methods: Different solvent extracts of Liquidambar orientalis Mill resin were prepared. The cytotoxicity of extracts was determined using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide) assay. HeLa (Human cervix adenocarcinoma), A-549 (Human alveolar adenocarcinoma), MCF-7 (Human breast adenocarcinoma), CaCo-2 (Human colon colorectal adenocarcinoma), mPANC96 (Human pancreas adenocarcinoma), PC-3 (Human prostate adenocarcinoma), U87MG (Human glioblastoma-astrocytoma) and as a normal cell line HEK293 (Human embryonic kidney cells) and Vero (African green monkey kidney epithelial cells) were used for testing cytotoxicity. RAW 264.7 (murine macrophage cell lines) was used to determine the inhibition levels of inducible nitric oxide synthase (iNOS). HL-60 (human acute myeloid leukemia) was used to determine antioxidant activity as DCF production per cent. Results: Hexane, dichloromethane, methanol and water extracts were prepared, and their iNOS inhibitory, cytotoxic and antioxidant activity were investigated. The estimated IC 50 values of extracts varied from 6.68 to 48.90 mu g/ml after treatment with different doses of extracts for 48 h. Inhibition of the hexane, dichloromethane, methanol, and water extracts on LPS-induced NO production in RAW 264.7 macrophage were showed that the all extracts inhibited NO production in activated RAW 264.7 cells, except methanol extract. Hexane, dichloromethane and methanol extracts inhibited NO production with IC 50 values of 22 mu g/ml, 22 mu g/ml, and 21/ml mu g, respectively. The antioxidant activity was determined by the production of DCF in HL-60 cells. The extracts showed a potent antioxidant effect, with an IC 50 of in between from 19.01 to 39.77 mu g/ml. Conclusion: This study is the first report showing the potential of Liquidambar orientalis Mill resin extracts for cytotoxicity, iNOS inhibition and the antioxidant activity as an alternative therapeutic approach for traditional uses. The results demonstrated that Liquidambar orientalis dichloromethane resin extracts showed strongest cytotoxic effect while all extracts except methanolic extracts exhibited moderate iNOS inhibition. All extracts other than hexane have a potent antioxidant effect in the cellular-based assay. In conclusion, further studies should focus on the purification of the active components of this extracts and to investigate the possible mode of action to obtain a better understanding of their potential use as cytotoxic, anti-inflammatory and antioxidant agents.en_US
dc.identifier.doi10.1515/tjb-2016-0030
dc.identifier.endpage205en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issn0250-4685en_US
dc.identifier.issn1303-829Xen_US
dc.identifier.issue3en_US
dc.identifier.startpage198en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2016-0030
dc.identifier.urihttps://hdl.handle.net/11454/52751
dc.identifier.volume41en_US
dc.identifier.wosWOS:000377541000009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHamamelidaceaeen_US
dc.subjectLiquidambar orientalis Millen_US
dc.subjectcytotoxic activityen_US
dc.subjectiNOS inhibitoryen_US
dc.subjectantioxidant activityen_US
dc.titleAntioxidant, cytotoxic and iNOS activity of Liquidambar orientalis Mill. resin extractsen_US
dc.typeArticleen_US

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