Isolation of hydroxytyrosol from olive leaves extract, radioiodination and investigation of bioaffinity using in vivo/in vitro methods

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dc.contributor.authorOzkan, M.
dc.contributor.authorMuftuler, F. Z. Biber
dc.contributor.authorKilcar, A. Yurt
dc.contributor.authorMedine, E. I.
dc.contributor.authorUnak, P.
dc.date.accessioned2019-10-27T21:55:40Z
dc.date.available2019-10-27T21:55:40Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractIt is known that medicinal plants like olive have biological activities due to their flavonoid content such as olueropein, tyrosol, hydroxytyrosol etc. In current study, hydroxytrosol (HT) which is one of the major phenolic compounds in olive, olive leaves and olive oil, was isolated after methanol extraction and purification of olive leaves which are grown in the northern Anatolia region of Turkey. The isolated HT was radiolabeled with I-131 (I-131-HT) and the bioaffinity of this radiolabeled component of olive leaves extract was investigated by using in vivo/in vitro methods. It was found that HT could be radiolabeled with I-131 in yields of 95.6 +/- 4.4% (n = 8), and in vivo studies showed that I-131-HT is taken up by urinary bladder, stomach, small intestine, large intestine, breast and prostate. Significant incorporation of activity was observed in cell lines via in vitro studies.en_US
dc.description.sponsorshipEge UniversityEge University [2012 NBE 010]en_US
dc.description.sponsorshipThis work is supported by Ege University Research Fund (contract no 2012 NBE 010). The authors thank MSc student Hasan Sezer Sezgin and PhD student Betul Cekic for the technical assistance during the animal experiments.en_US
dc.identifier.doi10.1524/ract.2013.2068
dc.identifier.endpage593en_US
dc.identifier.issn0033-8230
dc.identifier.issn0033-8230en_US
dc.identifier.issue9en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage585en_US
dc.identifier.urihttps://doi.org/10.1524/ract.2013.2068
dc.identifier.urihttps://hdl.handle.net/11454/48256
dc.identifier.volume101en_US
dc.identifier.wosWOS:000325508900007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofRadiochimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOlive leaves extracten_US
dc.subjectHydroxytyrosol (HT)en_US
dc.subjectRadioiodinated hydroxytyrosol (I-131-HT)en_US
dc.subjectBiodistributionen_US
dc.subjectHutu-80 cellsen_US
dc.subjectCaco-2 cellsen_US
dc.subjectPC-3 cellsen_US
dc.subjectMCF-7 cellsen_US
dc.titleIsolation of hydroxytyrosol from olive leaves extract, radioiodination and investigation of bioaffinity using in vivo/in vitro methodsen_US
dc.typeArticleen_US

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