Formulation of organic and inorganic hydrogel matrices for immobilization of ß-glucosidase in microfluidic platform

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dc.contributor.authorKazan A.
dc.contributor.authorHeymuth M.
dc.contributor.authorKarabulut D.
dc.contributor.authorAkay S.
dc.contributor.authorYildiz-Ozturk E.
dc.contributor.authorOnbas R.
dc.contributor.authorMuderrisoglu C.
dc.contributor.authorSargin S.
dc.contributor.authorHeils R.
dc.contributor.authorSmirnova I.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2019-10-27T08:03:48Z
dc.date.available2019-10-27T08:03:48Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to formulate silica and alginate hydrogels for immobilization of ß-glucosidase. For this purpose, enzyme kinetics in hydrogels were determined, activity of immobilized enzymes was compared with that of free enzyme, and structures of silica and alginate hydrogels were characterized in terms of surface area and pore size. The addition of polyethylene oxide improved the mechanical strength of the silica gels and 68% of the initial activity of the enzyme was preserved after immobilizing into tetraethyl orthosilicate–polyethylene oxide matrix where the relative activity in alginate beads was 87%. The immobilized ß-glucosidase was loaded into glass–silicon–glass microreactors and catalysis of 4-nitrophenyl ß-d-glucopyranoside was carried out at various retention times (5, 10, and 15 min) to compare the performance of silica and alginate hydrogels as immobilization matrices. The results indicated that alginate hydrogels exhibited slightly better properties than silica, which can be utilized for biocatalysis in microfluidic platforms. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.description.sponsorship113M050 Bundesministerium für Bildung und Forschung: 01DL14002en_US
dc.description.sponsorshipFinancial supports provided by The Research and Technological Council of Turkey (TUBITAK) (113M050) and Bundesministerin f?r Bildung und Forschung (BMBF) (01DL14002) are highly appreciated. --en_US
dc.identifier.doi10.1002/elsc.201600218
dc.identifier.endpage722en_US
dc.identifier.issn1618-0240
dc.identifier.issn1618-0240en_US
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage714en_US
dc.identifier.urihttps://doi.org/10.1002/elsc.201600218
dc.identifier.urihttps://hdl.handle.net/11454/25418
dc.identifier.volume17en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-VCH Verlagen_US
dc.relation.ispartofEngineering in Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzymeen_US
dc.subjectHydrogelsen_US
dc.subjectImmobilizationen_US
dc.subjectMicroreactoren_US
dc.titleFormulation of organic and inorganic hydrogel matrices for immobilization of ß-glucosidase in microfluidic platformen_US
dc.typeArticleen_US

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