Production of extracellular aspartic protease in submerged fermentation with Mucor mucedo DSM 809

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dc.contributor.authorYegin, Sirma
dc.contributor.authorFernandez-Lahore, Marcelo
dc.contributor.authorGuvenc, Ulgar
dc.contributor.authorGoksungur, Yekta
dc.date.accessioned2019-10-27T21:14:50Z
dc.date.available2019-10-27T21:14:50Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractFungal milk-clotting enzymes have gained value as bovine Chymosin substitutes in the cheese industry. In this work, the effects of culture conditions on the production of extracellular milk clotting enzymes from Mucor mucedo DSM 809 in submerged fermentation were studied. The maximum activity was observed after 48 h of cultivation at 24 degrees C in Erlenmeyer flasks. The optimized initial pH and shaking speed for enzyme production were 4.5 and 220 rpm, respectively. Glucose at a concentration of 1% (w/ v) was the best carbon source for the production of enzyme among the carbohydrates examined (glucose, fructose, lactose, maltodextrin). On the other hand casein at a concentration of 0.5% (w/v) was the selected nitrogen source in the media formulation. Under optimized conditions enzyme levels reached 130 SU per ml fermentation broth. The inoculum type and size has also affected biomass production and the biosynthesis of the enzyme. The preferred method was the inoculation of the culture media with spores at a total load of 6x10(5) spores per flask.en_US
dc.description.sponsorshipBMBF-ERA-NetFederal Ministry of Education & Research (BMBF); Euro TransBio-3; [BIO-010103441508]; [JUB 5130-50290]en_US
dc.description.sponsorshipThis work was supported by BMBF-ERA-Net, euro TransBio-3, BIO-010103441508 (JUB 5130-50290).en_US
dc.identifier.endpage6386en_US
dc.identifier.issn1684-5315
dc.identifier.issn1684-5315en_US
dc.identifier.issue38en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage6380en_US
dc.identifier.urihttps://hdl.handle.net/11454/43449
dc.identifier.volume9en_US
dc.identifier.wosWOS:000282232900021en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofAfrican Journal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMilk clotting enzymeen_US
dc.subjectAspartic proteaseen_US
dc.subjectMucor mucedoen_US
dc.subjectSub-merged fermentationen_US
dc.titleProduction of extracellular aspartic protease in submerged fermentation with Mucor mucedo DSM 809en_US
dc.typeArticleen_US

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