In vitro effects of antibiofilm agents and antibiotics on coagulase-negative staphylococci

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dc.contributor.authorOzturk, Ismail
dc.contributor.authorTekintas, Yamac
dc.contributor.authorTemel, Aybala
dc.contributor.authorErmertcan, Safak
dc.contributor.authorKurutepe, Semra
dc.contributor.authorHosgor Limoncu, Mine
dc.date.accessioned2021-05-03T20:38:36Z
dc.date.available2021-05-03T20:38:36Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractCoagulase negative staphylococci (CoNS) are important nosocomial pathogens that cause biofilm infections. Biofilm provides advantages for microorganisms to resist antibiotics and host immune systems. Considering the increased antibiotic resistance, alternative treatments are needed to combat biofilm infections. in the present study, the effects of antibiotics including gentamicin (GEN), ciprofloxacin, doxycycline (DOX), rifampicin (RIF) and antibiofilm agents including N-acetylcysteine (NAC), ethylenediaminetetraaceticacid (EDTA), nisin (NIS), farnesol (FAR) on clinical CoNSbiofilm and IS256, icaA gene expression levels were evaluated. Forty-five CoNS strains were isolated from patients' catheters, at Manisa Celal Bayar University Hospital. The minimum inhibitory concentrations (MICs) of agents were detected by broth microdilution method with European Committee for Antimicrobial Susceptibility Testing (EUCAST) criteria. The combined effects of agents were investigated by checkerboard method. The antibiofilm effects of combinations were investigated by spectrophotometric microplate method. The effects of combinations on IS256 and icaA gene expressions were evaluated by real-time quantitative reverse-transcriptase PCR. Twenty-four isolates (53.3%) were detected as strong biofilm producer. Biofilm production was inhibited in seven isolates in the presence of EDTA+RIF and NIS+DOX while NIS+GEN combination and RIF inhibited biofilm in six isolates. Nine combinations were found to have synergistic effect against isolate #6 which are resistant to four different antibiotics. The expressions of icaA and IS256 were downregulated in the presence of EDTA, NAC+CIP, NAC+GEN, NIS+GEN, FAR+GEN. Antibiofilm agent/antimicrobial combinations could have promising effects for preventing catheter colonization. The further studies on antibiofilm treatment strategies would be beneficial for decreasing morbidity-mortality rates and healthcare costs caused by biofilms.en_US
dc.description.sponsorshipEge University Scientific Research Projects (BAP) commission [16-ECZ-019]en_US
dc.description.sponsorshipThis work was supported by the Ege University Scientific Research Projects (BAP) commission with 16-ECZ-019 project code.en_US
dc.identifier.doi10.35333/jrp.2020.241
dc.identifier.endpage832en_US
dc.identifier.issn2630-6344
dc.identifier.issn2630-6344en_US
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85097289133en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage821en_US
dc.identifier.urihttps://doi.org/10.35333/jrp.2020.241
dc.identifier.urihttps://hdl.handle.net/11454/70494
dc.identifier.volume24en_US
dc.identifier.wosWOS:000592829900004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMarmara Univen_US
dc.relation.ispartofJournal of Research in Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCoagulase negative staphylococcien_US
dc.subjectbiofilm productionen_US
dc.subjectantibiofilm agentsen_US
dc.subjectsynergistic effecten_US
dc.subjectgene expressionen_US
dc.titleIn vitro effects of antibiofilm agents and antibiotics on coagulase-negative staphylococcien_US
dc.typeArticleen_US

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