Molecular prevalence and genetic diversity of Hepatozoon spp. in stray cats of İzmir, Türkiye

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dc.authorscopusid58559177500
dc.authorscopusid57221117593
dc.authorscopusid25825299300
dc.authorscopusid56495590200
dc.authorscopusid57201674137
dc.authorscopusid57212213434
dc.authorscopusid23767084500
dc.contributor.authorKoçkaya, E.S.
dc.contributor.authorGüvendi, M.
dc.contributor.authorKöseoglu, A.E.
dc.contributor.authorKarakavuk, M.
dc.contributor.authorDeğirmenci, Döşkaya, A.
dc.contributor.authorErkunt, Alak, S.
dc.contributor.authorDöskaya, M.
dc.date.accessioned2024-08-25T18:32:26Z
dc.date.available2024-08-25T18:32:26Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractHepatozoon spp. are an apicomplexan protozoan parasites that infect vertebrates including mammals, marsupials, birds, reptiles, and amphibians. Among Hepatozoon species, H. canis and H. felis are causative agents of hepatozoonosis in dogs and cats, respectively and have veterinary importance. This study aimed to determine the prevalence of Hepatozoon spp. in stray cats living in İzmir and investigate genetic diversity among positive samples. To achieve this aim, the prevalence of Hepatozoon spp. 18S rRNA gene was screened by PCR in DNA samples extracted from blood samples of stray cats (n = 1012). Then, Hepatozoon-positive samples were sequenced and the generated data were used for species identification, phylogenetic and haplotype analyses. According to the results, among the samples screened, 2.37 % (24/1012) of them were found to be Hepatozoon-positive, and of these positive samples, 18 (18/24; 75 %) were successfully sequenced. BLAST and phylogenetic analyses revealed that all of these samples were H. felis. Also, phylogenetic analysis showed that H. felis samples were genotype I. Within H. felis samples isolated from cats living in different countries/regions, 9 haplotypes were detected and among these haplotypes, H-1 was found to be prevalent (n = 20 H. felis isolates). In conclusion, this study showed that the prevalence of Hepatozoon spp. was low in stray cats analyzed. Also, H. felis genotype I was predominant in comparison to other Hepatozoon species. © 2023 Elsevier Ltden_US
dc.description.sponsorshipEge Üniversitesi: TGA-2020-21040en_US
dc.description.sponsorshipThis study was partially supported by a project given by the Ege University Scientific Research Projects Coordination Unit (Project no.: TGA-2020-21040 ) to H.C.en_US
dc.identifier.doi10.1016/j.cimid.2023.102060
dc.identifier.issn0147-9571
dc.identifier.scopus2-s2.0-85169506110en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.cimid.2023.102060
dc.identifier.urihttps://hdl.handle.net/11454/100238
dc.identifier.volume101en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofComparative Immunology, Microbiology and Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectGenotype Ien_US
dc.subjectGenotypingen_US
dc.subjectHepatozoon felisen_US
dc.subjectSequencingen_US
dc.subjectStray catsen_US
dc.titleMolecular prevalence and genetic diversity of Hepatozoon spp. in stray cats of İzmir, Türkiyeen_US
dc.typeArticleen_US

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