EFFECTS OF DIMETHYL SULFOXIDE (DMSO) AND THAWING RATES ON SPERM MOTILITY FOR CRYOPRESERVATION OF SPERM IN GILTHEAD SEABREAM (SPARUS AURATA)
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of the present study was to establish a protocol for cryopreservation of sperm in Sparus aurata and also to verify the applicability under culture conditions. Cryopreservation of sperm was attempted by using two extenders: Extender A (10 % egg yolk, buffer solution) and Extender B (1% NaCl, 10% BSA Bovine Serum Albumin). Cryoprotectants were assessed at three different concentrations (5, 10 and 15 %, DMSO) individually as well as in combination with varying equilibration times (10 and 30 min). in order to maintain freezing rate, freezing protocols were adjusted by -30 degrees C/min intervals. Obtained results indicated that cryomedium constituting of Extender A with addition of 10% DMSO in dilution ratio of 1:3 (sperm:cryomedium) at an equilibration time of and freezing rate of -30 degrees C/min was more desirable compared to the other experimental procedures (Extender B) that were assessed (P>0.05). Moreover, 40 degrees C for 10 s presented highest post-thaw motility after end of the first month (72,7 +/- 0,35%) (P>0.05). Finally, it is determined that Extender A exhibited better performance in terms of sperm motility, freezing and thawing rate and also this ratio could be effectively utilized for cryopreservation of sperm for this species.
Açıklama
Anahtar Kelimeler
Gilthead Sea Bream, Sperm, Motility, Cryopreservation
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
29
Sayı
4A
