Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook

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dc.contributor.authorEmir, Ahmet
dc.contributor.authorEmir, Ceren
dc.contributor.authorBozkurt, Buket
dc.contributor.authorOnur, Mustafa Ali
dc.contributor.authorSomer, Nehir Unver
dc.contributor.authorKaya, Gulen Irem
dc.date.accessioned2019-10-27T11:21:59Z
dc.date.available2019-10-27T11:21:59Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut (R)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5,v/v/v) applied at a flow rate 1 mL min(-1) using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise.en_US
dc.description.sponsorshipEge University Research FundEge University [09/ECZ/037]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [104T272]; EBILTEMEge University [2007/BIL/007]en_US
dc.description.sponsorshipThis study was financially supported by the Ege University Research Fund (Project No: 09/ECZ/037) and partially supported by TUBITAK (104T272) and EBILTEM (2007/BIL/007).en_US
dc.identifier.doi10.1590/s2175-97902017000115063
dc.identifier.issn1984-8250
dc.identifier.issn2175-9790
dc.identifier.issn1984-8250en_US
dc.identifier.issn2175-9790en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1590/s2175-97902017000115063
dc.identifier.urihttps://hdl.handle.net/11454/32984
dc.identifier.volume53en_US
dc.identifier.wosWOS:000400155300003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Sao Paulo, Conjunto Quimicasen_US
dc.relation.ispartofBrazilian Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlkaloidsen_US
dc.subjectAmaryllidaceaeen_US
dc.subjectPlant extractsen_US
dc.subjectHigh Performance Liquid Chromatography/method validationen_US
dc.subjectHigh Perfomance Liquid Chromatography/quantitative analysisen_US
dc.titleApplication of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hooken_US
dc.typeArticleen_US

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