Screening the antimicrobial, cytotoxic and hemolytic effects of skin-parotoid gland secretions of 13 taxa from six species and one hybrid population of Anatolian endemic Lycian salamanders (Genus: Lyciasalamandra )

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dc.contributor.authorKaris, Mert
dc.contributor.authorYalcin, H. Tansel
dc.contributor.authorGocmen, Bayram
dc.contributor.authorNalbantsoy, Ayse
dc.date.accessioned2024-08-31T07:46:57Z
dc.date.available2024-08-31T07:46:57Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground and purpose: The diverse bioactive content in the skin and parotoid glands of amphibians makes them unique and highly potential sources for pharmacological developments. The main aim of the study is to determine the total protein amounts, in vitro cytotoxicities, antimicrobial activities and hemolytic effects of skin-parotoid secretions of these endemic Lycian salamanders (Genus: Lyciasalamandra ). Materials and methods: Lycian salamander specimens of 13 taxa from six species and one hybrid population are collected from southwestern Anatolia. Skin-parotoid secretions were obtained, clarified, supernatants snapfrozen then lyophilized. Total protein amounts were determined by BCA assay kit. The cytotoxicity was determined on eight different cell lines (one healthy and seven cancerous) using MTT assay. The antimicrobial activity was assesed using 11 different microorganisms (nine bacteria and two yeast) from six genus by MIC method. Hemolytic effects were measured on rabbit red blood cells. Results: Lycian salamanders'skin-parotoid gland secretions showed variable cytotoxic effects on all cell lines with IC 50 values from 5.41 +/- 0.17 mu g/ml to ineffective. MIC results of antimicrobial activity tests were also variable and from 3.9 mu g/mL to non -effective. Studied skin-parotoid secretions have no hemolytic activities on rabbit red blood cells at concentrations of 0.5, 5 and 50 mu g/mL. Conclusions: Skin-parotoid gland secretions of Anatolian endemic Lycian salamanders have sufficient and remarkable therapeutic potential to be candidate sources of natural and biologically active substances for novel drug discoveries.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK) [114Z599]en_US
dc.description.sponsorshipWe would like to extend our thanks to AREL (Research and Education Laboratory. Ege University School of Medicine) for usage of their laboratory facilities during the study. This paper is dedicated to the late Prof. Dr. Bayram GOECMEN, who was the PhD supervisor of the senior author (MK) , a valuable zoologist and a passionate nature lover, who passed away on 22 March 2019. Research Funding: Scientific and Technical Research Council of Turkey (TUBITAK) Grant No: 114Z599.en_US
dc.identifier.doi10.18054/pb.v125i3-4.26104
dc.identifier.endpage194en_US
dc.identifier.issn0031-5362
dc.identifier.issue3-4en_US
dc.identifier.scopus2-s2.0-85197647946en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage187en_US
dc.identifier.urihttps://doi.org/10.18054/pb.v125i3-4.26104
dc.identifier.urihttps://hdl.handle.net/11454/104262
dc.identifier.volume125en_US
dc.identifier.wosWOS:001265021800005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPeriodicum Biologorumen_US
dc.relation.ispartofPeriodicum Biologorumen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectAmphibian Skin Secretionen_US
dc.subjectCytotoxicityen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectHemolytic Effecten_US
dc.subjectLyciasalamandraen_US
dc.subjectAnatoliaen_US
dc.subjectEndemicen_US
dc.titleScreening the antimicrobial, cytotoxic and hemolytic effects of skin-parotoid gland secretions of 13 taxa from six species and one hybrid population of Anatolian endemic Lycian salamanders (Genus: Lyciasalamandra )en_US
dc.typeArticleen_US

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