Detection of benzoic acid by an amperometric inhibitor biosensor based on mushroom tissue homogenate

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dc.contributor.authorSezginturk, MK
dc.contributor.authorGoktug, T
dc.contributor.authorDinckaya, E
dc.date.accessioned2019-10-27T19:22:55Z
dc.date.available2019-10-27T19:22:55Z
dc.date.issued2005
dc.departmentEge Üniversitesien_US
dc.description.abstractAn amperometric benzoic acid-sensing inhibitor biosensor was prepared by immobilizing mushroom (Agaricus bisporus) tissue homogenate on a Clark-type oxygen electrode. The effects of the quantity of mushroom tissue homogenate, the quantity of gelatin and the effect of the crosslinking agent glutaraldehyde percent on the biosensor were studied. The optimum concentration of phenol used as substrate was 200 mu M. The bioanalytical properties of the proposed biosensor, such as dependence of the biosensor response on the pH value and the temperature, were investigated. The biosensor responded linearly to benzoic acid in a concentration range of 25-100 mu M. Standard deviation (s.d.) was 0.49 mu M for 7 successive determinations at a concentration of 75 PM. The inhibitor biosensor based on mushroom tissue homogenate was applied for the determination of benzoic acid in fizzy lemonade, some fruits and groundwater samples. Results were compared to those obtained using AOAC method, showing a good agreement.en_US
dc.identifier.endpage334en_US
dc.identifier.issn1330-9862
dc.identifier.issn1334-2606
dc.identifier.issn1330-9862en_US
dc.identifier.issn1334-2606en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage329en_US
dc.identifier.urihttps://hdl.handle.net/11454/39134
dc.identifier.volume43en_US
dc.identifier.wosWOS:000234084600003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFaculty Food Technology Biotechnologyen_US
dc.relation.ispartofFood Technology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbenzoic aciden_US
dc.subjectinhibitor biosensorsen_US
dc.subjectpolyphenol oxidasesen_US
dc.subjectfood additivesen_US
dc.subjectClark-type oxygen electrodeen_US
dc.titleDetection of benzoic acid by an amperometric inhibitor biosensor based on mushroom tissue homogenateen_US
dc.typeArticleen_US

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