Examples of Using of Electrochemical Detection at Pencil Graphite Electrode with Enzymatic Labeling for Analysis of Nucleotide Sequence

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dc.contributor.authorPlucnara, Medard
dc.contributor.authorHaronikova, Lucia
dc.contributor.authorSpacek, Jan
dc.contributor.authorHavran, Ludek
dc.contributor.authorHorakova, Petra
dc.contributor.authorPivonkova, Hana
dc.contributor.authorEcsin, Ece
dc.contributor.authorErdem, Arzum
dc.contributor.authorFojta, Miroslav
dc.contributor.editorNavratil, T
dc.contributor.editorFojta, M
dc.contributor.editorSchwarzova, K
dc.date.accessioned2019-10-27T22:29:34Z
dc.date.available2019-10-27T22:29:34Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.descriptionInternational Conference on Modern Electrochemical Methods XXXV -- MAY 18-22, 2015 -- Jetrichovice, CZECH REPUBLICen_US
dc.description.abstractMany examples of utilization of enzymatic labeling for DNA sequence analysis has been described in literature so far. Some of them involve hybridization with complementary biotinylated probe, while others use incorporation of biotinylated nucleotides into DNA strand by DNA polymerases. Common approach is then binding of streptavidine-enzyme conjugates to biotin tags and incubation with substrate, which is converted to detectable product. Here, two recent applications using this principle are described for the detection of PCR amplicons and for SNP typing. Both techniques are combined with detection at pencil graphite electrodes.en_US
dc.description.sponsorshipCharles Univ, Fac Sci, Dept Analyt Chem, UNESCO Lab Environm Electrochemistry, Inst Biophysen_US
dc.identifier.endpage179en_US
dc.identifier.isbn978-80-905221-3-8
dc.identifier.startpage175en_US
dc.identifier.urihttps://hdl.handle.net/11454/51125
dc.identifier.wosWOS:000358792900040en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isocsen_US
dc.publisherLenka Srsenova-Best Servisen_US
dc.relation.ispartofXxxv Moderni Elektrochemicke Metodyen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzymatic labelingen_US
dc.subjectAlkaline phosphataseen_US
dc.subjectSingle nucleotide polymorphismen_US
dc.subjectPencil graphite electrodeen_US
dc.titleExamples of Using of Electrochemical Detection at Pencil Graphite Electrode with Enzymatic Labeling for Analysis of Nucleotide Sequenceen_US
dc.typeConference Objecten_US

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