Molecular evaluation of t(14;18)(bcl-2/IgH) translocation in follicular lymphoma at diagnosis using paraffin-embedded tissue sections [Foliküler lenfoma Tani{dotless}si{dotless}nda Parafine-Gömülü Dokular kullani{dotless}larak t(14;18)(bcl-2/IgH) translokasyonun moleküler yöntemler ile Degerlendirilmesi]

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dc.contributor.authorSelvi N.
dc.contributor.authorKosova B.
dc.contributor.authorHekimgil M.
dc.contributor.authorGündüz C.
dc.contributor.authorKaymaz B.T.
dc.contributor.authorKaraca E.
dc.contributor.authorSaydam, G..
dc.contributor.authorTombuloglu M.
dc.contributor.authorBüyükkeçeci F.
dc.contributor.authorÇagirgan S.
dc.contributor.authorErtan Y.
dc.contributor.authorTopçuoglu N.
dc.date.accessioned2019-10-26T21:48:07Z
dc.date.available2019-10-26T21:48:07Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: Follicular lymphoma (FL) is one of the most common lymphomas, and is characterized by t(14;18) (q32;q21) in more than 80% of patients. The aim of this study was to determine the rate of t(14;18) positivity based on the detection of mbr or mcr in paraffin-embedded tissue samples. Material and Methods: The study included 32 paraffin-embedded tissue samples collected from 32 consecutive FL patients that were diagnosed and followed-up at our hospital between 1999 and 2006. The MBR breakpoint was identified based on real-time PCR using a LightCycler v.2.0 t(14;18) Quantification Kit (MBR), multiplex PCR, and seminested PCR. To identify the mcr breakpoint, real-time PCR was performed using specific primers and the FastStart DNA Master SYBR Green I Kit. To detect t(14;18) via fluorescence in situ hybridization (FISH) nuclei from paraffin-embedded tissue sections were extracted and used together with LSI IgH (immunoglobulin heavy chain) (spectrum green)/bcl-2 (B-cell leukemia-lymphoma 2) (spectrum orange) probes. Results: The DNA and nuclei isolation success rate for B5 formalin-fixed, paraffin-embedded tissue sections (n = 12) was 42% and 33%, respectively, versus 95% and 60%, respectively, for 20 tissue sections fixed in formalin only. In all, 24 paraffin-embedded tissue sections were analyzed and mbr positivity was observed in the DNA of 82.14% via seminested PCR, in 53.57% via multiplex PCR, and in 28.57% via real-time PCR. We did not detect mcr rearrangement in any of the samples. In all, 15 of 16 patients (93.75%) whose nuclei were successfully isolated were observed to be t(14;18) positive via the FISH method. Conclusion: Semi-nested PCR and FISH facilitated the genetic characterization of FL tumors. As such, FISH and PCR complement each other and are both essential for detecting t(14;18) translocation.en_US
dc.identifier.doi10.5505/tjh.2012.93898
dc.identifier.endpage134en_US
dc.identifier.issn1300-7777
dc.identifier.issn1300-7777en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage126en_US
dc.identifier.urihttps://doi.org/10.5505/tjh.2012.93898
dc.identifier.urihttps://hdl.handle.net/11454/18642
dc.identifier.volume29en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Hematologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFISHen_US
dc.subjectFollicular lymphomaen_US
dc.subjectMultiplex PCRen_US
dc.subjectSemi-nested PCRen_US
dc.titleMolecular evaluation of t(14;18)(bcl-2/IgH) translocation in follicular lymphoma at diagnosis using paraffin-embedded tissue sections [Foliküler lenfoma Tani{dotless}si{dotless}nda Parafine-Gömülü Dokular kullani{dotless}larak t(14;18)(bcl-2/IgH) translokasyonun moleküler yöntemler ile Degerlendirilmesi]en_US
dc.typeArticleen_US

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