Synthesis and Cloning of a Small Bacillus Pheromone Gene (ComXRO-B-2) by Primer-Dimer Formation with PCR

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dc.contributor.authorDemir Dora D.
dc.contributor.authorKocagöz T.
dc.contributor.authorÖner F.
dc.date.accessioned2019-10-26T23:56:10Z
dc.date.available2019-10-26T23:56:10Z
dc.date.issued2008
dc.departmentEge Üniversitesien_US
dc.description.abstractComX pheromone is the major extracellular signaling peptide stimulating transformation in response to high cell density in Bacillus species. In this study, Bacillus mojavensis ComX(RO-B-2) pheromone gene was cloned to pGEMT-Easy vector based on TA cloning of PCR products. The gene encoding 11 amino acid peptides of Bacillus mojavensis RO-B-2 strain ComX pheromone (GLQIYTNWVPS) was obtained by PCR amplification of 2 primers complementary to each other at their 3' end. This eliminated the need for the original bacterial gene as DNA template for PCR. The amplified PCR products were ligated directly without any modification by T4 DNA ligase into pGEMT-Easy vector, which has a single overhanging T residue at the 3' ends of the cloning site.en_US
dc.identifier.endpage771en_US
dc.identifier.issn1300-0527
dc.identifier.issn1300-0527en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage765en_US
dc.identifier.urihttps://hdl.handle.net/11454/20757
dc.identifier.volume32en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus mojavensis RO-B-2en_US
dc.subjectComX pheromone geneen_US
dc.subjectPCRen_US
dc.subjectprimer-dimeren_US
dc.subjectTA cloningen_US
dc.titleSynthesis and Cloning of a Small Bacillus Pheromone Gene (ComXRO-B-2) by Primer-Dimer Formation with PCRen_US
dc.typeArticleen_US

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