Fermentation medium optimization for 1,3-propanediol production using taguchi and box-behnken experimental designs

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dc.contributor.authorGungormusler M.
dc.contributor.authorGonen C.
dc.contributor.authorOzdemir G.
dc.contributor.authorAzbar N.
dc.date.accessioned2019-10-26T22:17:38Z
dc.date.available2019-10-26T22:17:38Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractKlebsiella species are excellent 1,3-propanediol (1,3 PDO) producers. Klebsiella pneumoniae is a well-studied microorganism and, therefore, its metabolic pathway is known. Accordingly, a number of by-products are simultaneously produced together with 1,3 PDO. Optimization of medium composition significantly decreased these byproducts' concentrations. Optimization of culture media concentrations on 1,3 PDO production by K pneumoniae was studied using statistical experimental designs and analysis, namely, Taguchi Orthogonal Array (TOA) and BoxBehnken design (BBD). The optimal composition of the fermentation medium for maximum 1,3 PDO yield, with a L18 (2?1 × 3?7) design using TOA, and after that, a threelevel three-factor BBD, was obtained. The most significant chemicals were found to be glycerol (p<0.0001) and K2HPO4 (p<0.05). MgSO 4.7H2O, which is the third significant factor obtained from TOA, was not found to be significant (p>0.05) from BBD. A maximum 1,3 PDO concentration of 23.6 g/L was obtained which is higher compared to that foreseen by the statistical model (9.3 g/L). © by PSP.en_US
dc.identifier.endpage2847en_US
dc.identifier.issn1018-4619
dc.identifier.issn1018-4619en_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage2840en_US
dc.identifier.urihttps://hdl.handle.net/11454/19478
dc.identifier.volume19en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject1,3-propanediolen_US
dc.subjectBox-Behnken response surface methodologyen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectTaguchi orthogonal arrayen_US
dc.titleFermentation medium optimization for 1,3-propanediol production using taguchi and box-behnken experimental designsen_US
dc.typeArticleen_US

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