Affinity based laccase immobilization on modified magnetic nanoparticles: Biosensing platform for the monitoring of phenolic compounds

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dc.contributor.authorBabadostu A.
dc.contributor.authorGuldu O.K.
dc.contributor.authorDemirkol D.O.
dc.contributor.authorMedine E.I.
dc.contributor.authorUnak P.
dc.contributor.authorTimur S.
dc.date.accessioned2019-10-26T21:24:38Z
dc.date.available2019-10-26T21:24:38Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThe authors report an electrochemical phenol biosensor based on the immobilization of laccase (Lac) on the surface of copper capped magnetic core-shell (Fe3O4-SiO2) nanoparticles (MNPs). After synthesis, MNP surfaces were functionalized by silanization and then, modified with histidine (His) and copper, respectively. The performance of the MNP-His/Cu/Lac biosensor has been investigated at -0.05 V versus Ag/AgCl. The proposed biosensor allowed to detect catechol and phenol in the range of 0.01-0.4 mM and 0.025-0.2 mM, respectively. Finally, phenol analysis in culture medium of P. putida adapted to phenol was successfully demonstrated with appreciable recovery values. © 2015 Copyright Taylor & Francis Group, LLC.en_US
dc.description.sponsorshipCentral University Basic Scientific Research Business Expenses Special Funds: 2010 ILAM 03 Ege Üniversitesien_US
dc.description.sponsorshipThe authors thank Ege University Scientific Research Fund for the financial support with the Project Number 2010 ILAM 03. --en_US
dc.identifier.doi10.1080/00914037.2014.936598
dc.identifier.endpage266en_US
dc.identifier.issn0091-4037
dc.identifier.issn0091-4037en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage260en_US
dc.identifier.urihttps://doi.org/10.1080/00914037.2014.936598
dc.identifier.urihttps://hdl.handle.net/11454/17185
dc.identifier.volume64en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Inc.en_US
dc.relation.ispartofInternational Journal of Polymeric Materials and Polymeric Biomaterialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBioprocess monitoringen_US
dc.subjectbiosensoren_US
dc.subjectlaccaseen_US
dc.subjectmagnetic nanoparticlesen_US
dc.subjectphenolic compoundsen_US
dc.subjectsurface modificationen_US
dc.titleAffinity based laccase immobilization on modified magnetic nanoparticles: Biosensing platform for the monitoring of phenolic compoundsen_US
dc.typeArticleen_US

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