Nasal fluid sample as a reliable matrix for determination of cytokine levels in childhood asthma

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dc.authoridKartal Ozturk, Gokcen/0000-0002-0793-9710
dc.contributor.authorDoulatpanah, Mojtaba
dc.contributor.authorKocamanoglu, Meltem
dc.contributor.authorYıldırım Sözmen, Eser
dc.contributor.authorKartal Öztürk, Gökçen
dc.contributor.authorDemir, Esen
dc.contributor.authorGülen, Figen
dc.contributor.authorAkçay, Yasemin
dc.date.accessioned2024-08-25T18:32:46Z
dc.date.available2024-08-25T18:32:46Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractObjectives: Childhood asthma is a chronic disease with high incidence worldwide. As a lifelong disease, asthma has episodes. Inflammation continues to occur in the clinical remission of asthma. It can be difficult to diagnose childhood asthma, especially in clinical remission. We hypothesized that some cytokines secreted to nasal fluid from the airway during inflammation might help diagnose clinical remission of asthma. Moreover, sampling nasal fluid is an easy and non-invasive procedure, so it may be a preferable sampling method.Methods: We measured levels of some interleukins (ILs), which are IL-4, IL-5, IL-6, IL-12p70, IL-13, IL-33, granulocyte-macrophage colony-stimulating factor (GM-CSF), periostin and thymic stromal lymphopoietin (TSLP) by Luminex magnetic bead-based immunoassay in nasal fluid and in serum of asthmatic children in clinical remission.Results: We found that IL-5, IL-6, IL-33, and periostin had elevated levels in nasal fluid. IL-5 and IL-33 had increased levels in the nasal fluid of the patients with immunoglobulin E (IgE) high and low phenotypes. While the nasal fluid TSLP levels were positively correlated with most of the increased serum cytokine levels of non-allergic asthmatic children, the nasal fluid GM-CSF levels were positively correlated with most of the increased serum cytokine levels of the allergic asthmatic children.Conclusions: IL-5, IL-6, IL-33, and periostin had elevated levels in the nasal fluid of the patients in clinical remission. The nasal fluid GM-CSF levels of the allergic patients and nasal fluid TSLP levels of the non-allergic patients had a positive correlation with most of the serum cytokine levels. Thus, our results showed that nasal fluid might be a preferable biological sample to diagnose asthma in children.en_US
dc.description.sponsorshipOffice of Scientific Research Projects of Ege University [18-TIP-022]en_US
dc.description.sponsorshipThis study funded by Office of Scientific Research Projects of Ege University with the project ID 18-TIP-022.en_US
dc.identifier.doi10.1515/tjb-2022-0147
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.urihttps://doi.org/10.1515/tjb-2022-0147
dc.identifier.urihttps://hdl.handle.net/11454/100346
dc.identifier.wosWOS:001049806500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectbiomarkeren_US
dc.subjectchildhood asthmaen_US
dc.subjectcytokinesen_US
dc.subjectimmune responseen_US
dc.subjectremissionen_US
dc.subjectInflammationen_US
dc.subjectLymphopoietinen_US
dc.subjectPathogenesisen_US
dc.subjectExpressionen_US
dc.subjectImmunityen_US
dc.subjectRolesen_US
dc.subjectIl-33en_US
dc.titleNasal fluid sample as a reliable matrix for determination of cytokine levels in childhood asthmaen_US
dc.typeArticleen_US

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