Analysis of cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) gene related to neonatal isoerythrolysis in stray cats of Izmir, Turkey [Türkiye, İzmir sokak kedilerinde neonatal izoeritrolizisle ilişkili sitidin monofosfat-N-asetilnöraminik asit hidroksilaz (CMAH) geninin analizi]

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dc.contributor.authorCan H.
dc.contributor.authorAtalay Şahar E.
dc.contributor.authorDöşkaya M.
dc.contributor.authorÖzdemir H.G.
dc.contributor.authorCaner A.
dc.contributor.authorDeğirmenci Döşkaya A.
dc.contributor.authorGürüz Y.
dc.contributor.authorÜn C.
dc.date.accessioned2019-10-27T08:20:36Z
dc.date.available2019-10-27T08:20:36Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractNeonatal isoerythrolysis is a life threatening disease in new born cats. It occurs when type A or type AB kittens are born from a type B queen (female cat). A homozygous 18 bp insertion located in cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) gene has been detected in type B cats, causing production of inactive CMAH enzyme. Currently, molecular methods are being used to determine type B blood in female cats, which can help prevent neonatal isoerythrolysis in kittens. These molecular assays target the presence of 18 bp insertion in CMAH gene. In this study, we aimed to analyze the potential of neonatal isoerythrolysis among stray cats of İzmir, Turkey using PCR detecting the 18 bp insertion in CMAH gene. During the study, we analyzed 793 cats’ blood sample for the presence of 18 bp insertion in CMAH gene. Three cats known to have blood types A, B, and AB were used as control in PCR. According to the PCR results, blood type A control cat displayed a 175 bp product indicating a homozygous type A cat while blood type control B cat showed a 193 bp product in CMAH gene (with 18 bp insertion) indicating a homozygous type B cat. Interestingly, blood type AB control cat showed a heterozygous pattern for CMAH gene, in which three different bands (175 bp like that of type A, 193 bp product for type B, and the third unique band with approximately 240 bp size) were detected. Among 793 stray cats of İzmir, 791 were homozygous for CMAH gene with 175 bp band size (99.7%). The remaining two stray cats showed heterozygous band pattern like blood type AB cat (0.12%). Overall, 175 bp band displaying type A cats are prevalent contrary to the two cats that have type AB pattern and non-existence of homozygous type B cats. These results show that the potential of neonatal isoerythrolysis in stray cats of İzmir is minimal. Future studies are required to scrutinize the reason(s) for non-existence of type B cats in İzmir and presence of unique band in blood type AB. © 2016, Veteriner Fakultesi Dergisi. All rights reserved.en_US
dc.identifier.doi10.9775/kvfd.2015.14637
dc.identifier.endpage359en_US
dc.identifier.issn1300-6045
dc.identifier.issn1300-6045en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage355en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2015.14637
dc.identifier.urihttps://hdl.handle.net/11454/25723
dc.identifier.volume22en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherVeteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCaten_US
dc.subjectCMAH geneen_US
dc.subjectInsertionen_US
dc.subjectNeonatal isoerythrolysisen_US
dc.subjectPCRen_US
dc.titleAnalysis of cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) gene related to neonatal isoerythrolysis in stray cats of Izmir, Turkey [Türkiye, İzmir sokak kedilerinde neonatal izoeritrolizisle ilişkili sitidin monofosfat-N-asetilnöraminik asit hidroksilaz (CMAH) geninin analizi]en_US
dc.typeArticleen_US

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