Isolation and immobilization of his-tagged alcohol dehydrogenase on magnetic nanoparticles in one step: Application as biosensor platform

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dc.contributor.authorGuldu O.K.
dc.contributor.authorEce S.
dc.contributor.authorEvran S.
dc.contributor.authorMedine E.I.
dc.contributor.authorDemirkol D.O.
dc.contributor.authorUnak P.
dc.contributor.authorTimur S.
dc.date.accessioned2019-10-27T08:21:45Z
dc.date.available2019-10-27T08:21:45Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractHis-tagged Alcohol dehydrogenase was produced as a recombinant protein in E. coli. Afterwards, isolation and immobilization of the enzyme was carried in one-step via copper modified magnetic nanoparticles (MNPs) by the effect of interactions between Cu and histidine. The resulting enzyme bound MNPs was then attached to the surface of carbon paste electrode by the magnetic force and used as an electrochemical biosensor for the alcohol sensing applications. © 2014 Taylor and Francis Group, LLC.en_US
dc.identifier.doi10.1080/10601325.2014.936236
dc.identifier.endpage705en_US
dc.identifier.issn1060-1325
dc.identifier.issn1060-1325en_US
dc.identifier.issue9en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage699en_US
dc.identifier.urihttps://doi.org/10.1080/10601325.2014.936236
dc.identifier.urihttps://hdl.handle.net/11454/26099
dc.identifier.volume51en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Inc.en_US
dc.relation.ispartofJournal of Macromolecular Science, Part A: Pure and Applied Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectalcohol biosensingen_US
dc.subjectHis-tagged alcohol dehydrogenaseen_US
dc.subjectmagnetic nanoparticlesen_US
dc.titleIsolation and immobilization of his-tagged alcohol dehydrogenase on magnetic nanoparticles in one step: Application as biosensor platformen_US
dc.typeArticleen_US

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