Amino acid intercalated montmorillonite: electrochemical biosensing applications

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dc.contributor.authorDemir, Filiz
dc.contributor.authorDemir, Bilal
dc.contributor.authorYalcinkaya, Esra E.
dc.contributor.authorCevik, Serdar
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorAnik, Ulku
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T22:05:40Z
dc.date.available2019-10-27T22:05:40Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractThe present work is the first that includes the use of glycine (Gly), lysine (Lys) and glutamic acid (Glu) modified clay mineral matrices in the biosensors. For this purpose, initially, Gly, Lys and Glu were intercalated with montmorillonite (Mt), thus, various modified Mts were obtained. These modified materials were then characterized via X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric analysis (TGA/DTG) and scanning electron microscopy (SEM). In order to investigate the applicability of amino acid modified clay minerals in biosensor areas, glucose oxidase (GOx) was selected as the model enzyme and a GOx based biosensor was prepared. After immobilizing the enzyme with amino acid modified Mt onto a glassy carbon electrode, working conditions like pH and modifier type were optimized. Among the modified Mts, Gly-Mt was the optimum clay mineral type and pH 4.0 was the optimum pH value. Then analytical characteristics were examined under optimum experimental conditions. The linear range of optimum sensor design was 0.1-1.0 mM within the kinetic parameters of an immobilized enzyme K-m(app) m = 0.7 mM, I-max = 107.8 nA. Finally, the developed biosensor was applied to real samples where the results were compared with a spectrophotometric reference method.en_US
dc.identifier.doi10.1039/c4ra07026g
dc.identifier.endpage50113en_US
dc.identifier.issn2046-2069
dc.identifier.issn2046-2069en_US
dc.identifier.issue91en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage50107en_US
dc.identifier.urihttps://doi.org/10.1039/c4ra07026g
dc.identifier.urihttps://hdl.handle.net/11454/48544
dc.identifier.volume4en_US
dc.identifier.wosWOS:000343715000073en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofRsc Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleAmino acid intercalated montmorillonite: electrochemical biosensing applicationsen_US
dc.typeArticleen_US

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