Synthesis of alginate-silica hybrid hydrogel for biocatalytic conversion by beta-glucosidase in microreactor

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dc.contributor.authorOnbas, Rabia
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T09:59:21Z
dc.date.available2019-10-27T09:59:21Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractThe organic-inorganic hybrid materials have been used in different fields to immobilize biomolecules since they offer many advantages. The aim of this study was to optimize and characterize the alginate-silica hybrid hydrogel as a stable and injectable form for microfluidic systems using internal gelation method and increase the stability and activity of immobilized enzyme for biocatalytic conversions as well. Characterization was carried out by scanning electron microscopy, energy dispersive spectroscopy/mapping, Brunauer-Emmett-Teller, Barrett-Joyner-Halenda, and Fourier-transform infrared spectroscopy analyses, and the shrinkages of monoliths were evaluated. Subsequent to optimizing the enzyme concentration (40 mu g), hydrolytic conversion of 4-nitrophenyl beta-d-glucopyranoside (pNPG) was performed to understand the behavior of the bioconversion in the microfluidic system. The yield was 94% which reached the equilibrium at 24 h indicating that the alginate-silica gel derived microsystem overcome some drawbacks of monolithic systems. Additionally, bioconversion of Ruscus aculeatus saponins was carried out at the same setup in order to obtain aglycon part, which has pharmaceutical significance. Although pure aglycon could not be achieved, an intermediate compound was obtained based on the HPLC analysis. The developed formulation can be utilized for various life science applications.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey, TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113M050]; Ege University Research FundEge University [15-FBE-012]; TUBITAK 2210-C National Graduate Scholarship Programen_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey, TUBITAK (113M050), and Ege University Research Fund (15-FBE-012). The grant from TUBITAK 2210-C National Graduate Scholarship Program is highly appreciated. Additionally, the authors thank Dr. Ozgur Tag for his support in HPLC analysis.en_US
dc.identifier.doi10.1002/elsc.201800124
dc.identifier.endpage46en_US
dc.identifier.issn1618-0240
dc.identifier.issn1618-2863
dc.identifier.issn1618-0240en_US
dc.identifier.issn1618-2863en_US
dc.identifier.issue1en_US
dc.identifier.startpage37en_US
dc.identifier.urihttps://doi.org/10.1002/elsc.201800124
dc.identifier.urihttps://hdl.handle.net/11454/29607
dc.identifier.volume19en_US
dc.identifier.wosWOS:000454679700005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofEngineering in Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlginate-silica hybrid hydrogelen_US
dc.subjectEnzymatic hydrolysisen_US
dc.subjectInternal gelationen_US
dc.subjectMicroreactoren_US
dc.subjectSaponinen_US
dc.titleSynthesis of alginate-silica hybrid hydrogel for biocatalytic conversion by beta-glucosidase in microreactoren_US
dc.typeArticleen_US

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