Aptasensor platform based on carbon nanofibers enriched screen printed electrodes for impedimetric detection of thrombin

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dc.contributor.authorErdem, Arzum
dc.contributor.authorCongur, Gulsah
dc.contributor.authorMayer, Guenter
dc.date.accessioned2019-10-27T22:59:10Z
dc.date.available2019-10-27T22:59:10Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractHerein, impedimetric detection of THR could be achieved using an aptasensing platform based on carbon nano-fibers enriched screen-printed electrodes (CNF-SPE). The resistance to charge transfer (R-ct) was recorded using electrochemical impedance spectroscopy (EIS) technique before/after the immobilization of amino-modified DNA aptamer (APT) selective to thrombin (THR) onto the surface of CNF-SPEs and the specific interaction between APT and THR. The selectivity of the aptasensor was also tested in the presence of a random DNA oligonucleotide and a DNA aptamer that were different from THR specific APT. The impedimetric aptasensing of target protein was also explored in the fetal bovine serum (FBS) medium at different concentration levels of THR. Additionally, the selectivity of the aptasensor was tested against bovine serum albumin (BSA) and protein C (PC) in FBS medium. This CNF-SPE based aptasensor platform allows a reliable, sensitive and selective impedimetric monitoring of THR. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.jelechem.2015.10.002
dc.identifier.endpage19en_US
dc.identifier.issn1572-6657
dc.identifier.issn1873-2569
dc.identifier.issn1572-6657en_US
dc.identifier.issn1873-2569en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage12en_US
dc.identifier.urihttps://doi.org/10.1016/j.jelechem.2015.10.002
dc.identifier.urihttps://hdl.handle.net/11454/51736
dc.identifier.volume758en_US
dc.identifier.wosWOS:000367278400003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofJournal of Electroanalytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbon nanofibersen_US
dc.subjectScreen printed electrodesen_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectAptameren_US
dc.subjectThrombinen_US
dc.subjectFetal bovine serumen_US
dc.titleAptasensor platform based on carbon nanofibers enriched screen printed electrodes for impedimetric detection of thrombinen_US
dc.typeArticleen_US

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