Silica-based monoliths for enzyme catalyzed reactions in microfluidic systems with an emphasis on glucose 6-phosphate dehydrogenase and cellulase

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dc.contributor.authorYesil-Celiktas, Ozlem
dc.contributor.authorCumana, Sucre
dc.contributor.authorSmirnova, Irina
dc.date.accessioned2019-10-27T22:06:47Z
dc.date.available2019-10-27T22:06:47Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractAn efficient on-chip enzyme immobilized monolith microreactor for glucose 6-phosphate dehydrogenase and its extension to cellulase catalyzed reactions is reported. The monoliths were fabricated using the sol-gel technique, where two different silica precursors were investigated, tetraethoxysilane (TEOS) and ethylene glycol modified silane (EGMS) by determining the activity and aging of the enzymes in the gels. Subsequently catalytic reactions were conducted in continuous flow microreactors and the performance of the system was evaluated by applying various flow rates (5, 10, 15, 20 mu l/min). The addition of polyethylene oxide (PEO) reduced shrinkage of the gels during a period of 4 days after gel preparation suggesting a strengthened gel skeleton leading to a reduced channeling within the porous structure. In the microfluidic systems, the highest conversion rates achieved were 20.0% and 28.8% at a flow rate of 5 mu l/min with TEOS-PEO and EGMS-PEO gels. Continuous reaction exhibited better yield than batch-wise operation using same volume/enzyme ratios which can be attributed to favorable enzyme substrate affinities. However, increase in the flow rate resulted in reduced conversion rates due to lower residence times. Consequently, the applied technique not only provided shorter preparation times but also sustained the stability of immobilized enzymes. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipHamburg Landesexzellensinitiative (Project Synbio); Joachim Herz Stiftungen_US
dc.description.sponsorshipThis project was financially supported by Hamburg Landesexzellensinitiative (Project Synbio) and Joachim Herz Stiftung. The authors are grateful to the Institute of Microsystem Technology of the Technical University of Hamburg for the preparation of the microsystems used in this study.en_US
dc.identifier.doi10.1016/j.cej.2013.08.065
dc.identifier.endpage172en_US
dc.identifier.issn1385-8947
dc.identifier.issn1873-3212
dc.identifier.issn1385-8947en_US
dc.identifier.issn1873-3212en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage166en_US
dc.identifier.urihttps://doi.org/10.1016/j.cej.2013.08.065
dc.identifier.urihttps://hdl.handle.net/11454/48884
dc.identifier.volume234en_US
dc.identifier.wosWOS:000328588300021en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofChemical Engineering Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSol-gelen_US
dc.subjectMicroreactoren_US
dc.subjectMicrofluidicsen_US
dc.subjectDehydrogenaseen_US
dc.subjectCellulaseen_US
dc.subjectImmobilizationen_US
dc.titleSilica-based monoliths for enzyme catalyzed reactions in microfluidic systems with an emphasis on glucose 6-phosphate dehydrogenase and cellulaseen_US
dc.typeArticleen_US

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