Determination of SHV-2, SHV-5, SHV-12 and CTX-M-3 Extended-Spectrum Beta-Lactamases in Clinical Enterobacteriaceae Isolates: Scientific Letter

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dc.contributor.authorTasli, Huseyin
dc.contributor.authorBahar, I. Hakki
dc.date.accessioned2019-10-27T21:14:50Z
dc.date.available2019-10-27T21:14:50Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractExtended spectrum beta-lactamases (ESBLs) are common in our country as well as in the world. However, data about the molecular types of these enzymes are lacking in Turkey. ESBLs were initially determined commonly as TEM and SHV derivatives, but in recent years CTX-M enzymes have become more dominant. The aim of the study was to investigate of CTX-M and PER genes in Enterobacteriaceae isolates which were positive for ESBL and carried TEM and/or SHV genes, and to determine DNA sequences of all enzymes. Presence of CTX-M and PER beta-lactamases was investigated with polymerase chain reaction (PCR) method in 28 strains obtained from the Microbiology Laboratory of Dokuz Eylul University Medical School. PCR-restriction fragment lenght polymorphism (PCR-RFLP) and DNA sequence analysis were performed on PCR positive strains. While CTX-M gene was determined in three strains using PCR, PER gene was determined in none of the strains. All CTX-M enzymes were found to belong to CTX-M-1 group according to RFLP analysis. As a result of both strands DNA sequence analysis, SHV-2 in four strains, SHV-5 in 14 strains, SHV-12 in 10 strains and CTX-M-3 in three strains were determined.en_US
dc.identifier.doi10.5336/medsci.2009-15118
dc.identifier.endpage1706en_US
dc.identifier.issn1300-0292
dc.identifier.issn2146-9040
dc.identifier.issn1300-0292en_US
dc.identifier.issn2146-9040en_US
dc.identifier.issue5en_US
dc.identifier.startpage1701en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2009-15118
dc.identifier.urihttps://hdl.handle.net/11454/43448
dc.identifier.volume30en_US
dc.identifier.wosWOS:000284798000037en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isotren_US
dc.publisherOrtadogu Ad Pres & Publ Coen_US
dc.relation.ispartofTurkiye Klinikleri Tip Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnterobacteriaceaeen_US
dc.subjectbeta-lactamasesen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectrestriction analysisen_US
dc.subjectDNA sequence analysisen_US
dc.titleDetermination of SHV-2, SHV-5, SHV-12 and CTX-M-3 Extended-Spectrum Beta-Lactamases in Clinical Enterobacteriaceae Isolates: Scientific Letteren_US
dc.typeArticleen_US

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