EVALUATION OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS IN POECILIA RETICULATA CAUDAL FIBROBLAST CELL CULTURE EXPOSED TO DIMETHYL PHTHALATE (DMP)

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dc.contributor.authorGokce, Burak
dc.contributor.authorUcuncu, Sema Isisag
dc.date.accessioned2023-01-12T20:19:18Z
dc.date.available2023-01-12T20:19:18Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractDimethyl phthalate (DMP) is one of the widely used phthalates which could cause environmental pollution due to widespread use of plastics. DMP poses potential toxicity risk for environment and human health. However, risk analysis of DMP as an environmental contaminant and toxicological effects of DMP on aquatic habitats have not been adequately evaluated. In recent years, in vitro test using cell lines are widely used in toxicological evaluations and risk analysis of environmental pollutants. In this study, cytotoxicity, oxidative damage, antioxidant enzyme activities and relative gene expressions were investigated in Poecilia reticulata caudal fibroblast cells exposed to DMP. In our study it is found that lethal concentrations (LC50) of DMP for cells after 24 and 48 h of exposure were 0,33 and 0,34 mM. Cells were exposed to three different concentrations of DMP (0.1, 0.2 and 0.4 mM) for 24 and 48 h to investigate oxidative damage, antioxidant enzyme activity and gene expression. In all experimental groups MDA content was increased. The superoxide dismutase (SOD) activity was significantly higher in low and medium concentration when compared to control groups but decreased at high concentrations after exposure of DMP for 24 and 48h. The Catalase (CAT) activities increased in low and middle concentration groups but reduced in high concentration groups after 24 and 48 h DMP exposure but glutathione-S-transferase (GST) enzyme activities were increased all experimental groups. In addition gene expressions of these enzymes were found to be associated with enzyme activities in all experimental groups. All findings indicate that DMP could cause physiological effects and cellular damage in Poecilia reticulata caudal fibroblast cells by causing oxidative damage and disturbing the expression levels of antioxidant enzymes. The results might contribute to the identification of biomarkers to monitor phthalate pollution and in vitro methods could be used to evaluate the effects of DMP in aquatic habitats.en_US
dc.identifier.endpage7805en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issn1018-4619en_US
dc.identifier.issn1610-2304en_US
dc.identifier.issue6Ben_US
dc.identifier.startpage7798en_US
dc.identifier.urihttps://hdl.handle.net/11454/79087
dc.identifier.volume30en_US
dc.identifier.wosWOS:000670303700091en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDimethyl phthalate (DMP)en_US
dc.subjectEcotoxicologyen_US
dc.subjectin vitroen_US
dc.subjectPoecilia reticulata caudal fibroblast cell cultureen_US
dc.subjectoxidative stressen_US
dc.subjectgene expressionen_US
dc.subjectAquatic Organismsen_US
dc.subjectEstersen_US
dc.subjectLiveren_US
dc.subjectFishen_US
dc.subjectFateen_US
dc.subjectBiomarkersen_US
dc.subjectDismutaseen_US
dc.subjectResponsesen_US
dc.subjectDefenseen_US
dc.titleEVALUATION OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS IN POECILIA RETICULATA CAUDAL FIBROBLAST CELL CULTURE EXPOSED TO DIMETHYL PHTHALATE (DMP)en_US
dc.typeArticleen_US

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