Synthesis of radioiodinated thymoquinone glucuronide conjugated magnetic nanoparticle (125I-TQG-Fe3 O4) and its cytotoxicity and in vitro affinity
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İstanbul Medipol University
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of the current study is to develop solid, semisolid or liquid form of radionuclide labeled thymoquinone glucuronide conjugated magnetite nanoparticles those targets to the tumor for diagnosis and therapy of cancer. For this purpose, thymoquinone (TQ), a molecule present in the seeds of Nigella (Nigella sativa) used to enhance the affinity of the drug to the tumor cells. TQ was isolated from its microsomes and an enzymatic method applied to synthesize beta glucuronic acid derivatized thymoquinone glucuronide (TQG). TQG attached magnetic nanoparticles (TQG-Fe3 O4). TQG- Fe3 O4 were radiolabeled with 125I and (125I-TQG- Fe3 O4) its cytotoxic effect and in vitro affinity was investigated. The IC50 values of TQG- Fe3 O4 were found 27.31, 18.68, 11.88 (µg/mL) respectively 24, 48 and 72 hours against A549 cell line by WST-8 test as a colorimetric way. Incorporation ratios of TQG- Fe3 O4 with A549 cells is the highest levels. It is seen that TQG-Fe3O4 could inhibit the apoptosis on A549 cells but, there is no apoptotic effect of the samples on BEAS-2B cells. Size distribution, cellular uptake and toxicity characteristics of TQG- Fe3 O4 in this study maintains a useful targeted delivery system in lung cancer diagnosis and therapy. © 2018, İstanbul Medipol University. All rights reserved.
Açıklama
Anahtar Kelimeler
Beta glucuronidase, Fe3O4, Glucuronide derivative, Magnetic nanoparticles, Nigella thymoquinone (TQ), Radiolabeling with 125I
Kaynak
Acta Pharmaceutica Sciencia
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
56
Sayı
2
