Comparison of IEF patterns of sarcoplasmic proteins of fish from North Atlantic and Aegean Sea

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dc.contributor.authorAltinelataman, Can
dc.contributor.authorKuendiger, Rainer
dc.contributor.authorCakli, Sukran
dc.contributor.authorRehbein, Hartmut
dc.date.accessioned2019-10-27T20:48:28Z
dc.date.available2019-10-27T20:48:28Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractIdentification of processed fish lacking morphological characteristics is of growing importance in the times of increasing global trade and illegal fishery. Protein electrophoresis techniques, like isoelectric focusing (IEF) of water-soluble proteins, are fast and inexpensive tools for identification of raw fishery products. Protein profiles of raw fish or fillet of the same species were found to be remarkably constant. However, it cannot be excluded that protein patterns of fish of the same species from distant stocks may show some variation. The protein profiles of commercially important fish from different fishing grounds were determined by collecting samples of 38 fish species from Aegean Sea and Turkish fish farms and of five species from North Atlantic waters. The raw fillet of the fish was analysed by IEF of water-soluble (sarcoplasmic) proteins. Species-specific protein profiles were obtained suited for identification of raw fish or fillet. When protein patterns and pI values were compared between Mediterranean and North Atlantic fish of the respective species, only minor differences were observed. These differences were related to the intensity of protein bands, but not to the position within the pH gradient of the IEF gel. The pI values (isoelectric points) of the prominent protein bands of the fish were determined to be used as a database. (C) 2008 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.foodcont.2008.11.007
dc.identifier.endpage985en_US
dc.identifier.issn0956-7135
dc.identifier.issn1873-7129
dc.identifier.issn0956-7135en_US
dc.identifier.issn1873-7129en_US
dc.identifier.issue11en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage980en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodcont.2008.11.007
dc.identifier.urihttps://hdl.handle.net/11454/42715
dc.identifier.volume20en_US
dc.identifier.wosWOS:000266676200003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Controlen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFish species identificationen_US
dc.subjectIEFen_US
dc.subjectMediterranean Seaen_US
dc.titleComparison of IEF patterns of sarcoplasmic proteins of fish from North Atlantic and Aegean Seaen_US
dc.typeArticleen_US

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