How to interpret relationship between ischemic time and ischemia/reperfusion injury in langendorff perfused rat hearts [Langendorff-perfüze si{dotless}çan kalbinde i·skemi süresi ile i·skemi/reperfüzyon hasari{dotless} arasi{dotless}ndaki i·lişki: Degerlendirme kriterleri neler olmali{dotless}?]

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dc.contributor.authorErtuna E.
dc.contributor.authorTürkseven S.
dc.contributor.authorHayran H.M.
dc.contributor.authorSargon M.F.
dc.contributor.authorYasa M.
dc.date.accessioned2019-10-26T21:39:41Z
dc.date.available2019-10-26T21:39:41Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: Diversities in ischemia time and injury assessment criteria used in Langendorff perfused isolated heart models complicate evaluation of the results. In this study, relationship between myocardial functional recovery, tissue viability and ultrastructural changes following ischemia were investigated in order to determine criteria which should be used to make precise interpretations. Material and Methods: Hearts were perfused in the constant flow Langendorff mode and were subjected to either continuous perfusion (Control), or to 15 (Ischemia15), 30 (Ischemia30) or 45 (Ischemia45) minutes ischemia followed by 30 minutes reperfusion. Left ventricular developed pressure, maximum contraction and relaxation rates, heart rate, arrhythmia incidence and duration were evaluated. Coronary effluent and heart cross-sections were used for creatine kinase-MB (CK-MB) measurements and morphological studies, respectively. Results: All functional parameters remained unchanged in Control and Ischemia15 groups. In Ischemia30 group, LVDP, +dP/dtmax and -dP/dtmax declined in early reperfusion, +dP/dtmax returned to basal values and heart rate declined at the end of reperfusion. In Ischemia45 group, all functional parameters except for heart rate declined in early reperfusion and returned to basal values at the end of reperfusion. Arrhythmia scores increased in all hearts subjected to ischemia. CK-MB was not elevated and triphenyltetrasolium staining showed that tissues were viable in Control, Ischemia15 and Ischemia30 groups. Necrotic fields were found and CK-MB increased in Ischemia45 group. Despite low myofibril scores, most marked ultrastructural pathological changes were found in Ischemia15 group. As ischemia time increased, the ultrastructural changes expressed as edema, glycogen, mitochondria and nuclei scores decreased. Conclusion: Our findings show that functional parameters should be evaluated in conjunction with morphological and biochemical data to make precise interpretation in ischemia reperfusion studies in isolated Langendorff perfused rat hearts. © 2013 by Türkiye Klinikleri.en_US
dc.identifier.doi10.5336/medsci.2012-30601
dc.identifier.endpage500en_US
dc.identifier.issn1300-0292
dc.identifier.issn1300-0292en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage493en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2012-30601
dc.identifier.urihttps://hdl.handle.net/11454/18197
dc.identifier.volume33en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkiye Kliniklerien_US
dc.relation.ispartofTurkiye Klinikleri Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHearten_US
dc.subjectIschemiaen_US
dc.subjectRatsen_US
dc.subjectReperfusion injuryen_US
dc.subjectUltrastructureen_US
dc.titleHow to interpret relationship between ischemic time and ischemia/reperfusion injury in langendorff perfused rat hearts [Langendorff-perfüze si{dotless}çan kalbinde i·skemi süresi ile i·skemi/reperfüzyon hasari{dotless} arasi{dotless}ndaki i·lişki: Degerlendirme kriterleri neler olmali{dotless}?]en_US
dc.typeArticleen_US

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