Thrombin immobilization to enzymatic modified PET and PAN fabrics and their applications

Basit Öğe Kaydı
dc.contributor.authorAkkaya, Alper
dc.contributor.authorPazarlioglu, Nurdan Kasikara
dc.date.accessioned2019-10-27T21:41:03Z
dc.date.available2019-10-27T21:41:03Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractEnzymatic modification of synthetic materials has immense potential both of the functionalization of polymeric materials, such as poly(acrylonitrile) or polyesters, and the production of polymers for special applications, such as medical devices and enzyme immobilization. In this study, poly(ethyleneterephtalate) and poly(acrylonitrile) fabrics were modified with commercial laccase and nitrilase, respectively. Contact angles of enzymatic modified and unmodified fabrics were measured and it was found contact angles of enzymatic modified fabrics were less than those of unmodified fabrics. Attenuated-Total-Reflection-Fourier-Transform infrared spectroscopy showed that carboxylic acid groups occurred on fabrics after enzymatic modifications. Surfaces of modified and unmodified fabrics were investigated using scanning electron microscopy. Surfaces of unmodified fabrics were smooth but surfaces of modified fabrics were rugged and cracked. Thrombin was immobilized in modified fabrics by using 1-Ethyl-3-(3-dimetylaminopropyl)-carbodiimide. Optimization studies were also performed for the immobilization of thrombin. After prepared material was tested to stop bleeding in vitro conditions and it was found that thrombin immobilized poly(ethyleneterephtalate) and poly(acrylonitrile) fabrics had a reduced recalcification time to 51 % and 89 %, respectively. Thrombin immobilized poly(ethyleneterephtalate) fabric was also tested in in vivo conditions by using Cavia porcellus and it was observed that this material caused bleeding to stop at a ratio of 24.6 %. The results were statistically significant.en_US
dc.description.sponsorshipEge University Research FoundationEge University [2007 FEN 010]en_US
dc.description.sponsorshipThis work was supported by Ege University Research Foundation 2007 FEN 010.en_US
dc.identifier.doi10.1007/s12221-012-0985-z
dc.identifier.endpage993en_US
dc.identifier.issn1229-9197
dc.identifier.issn1229-9197en_US
dc.identifier.issue8en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage985en_US
dc.identifier.urihttps://doi.org/10.1007/s12221-012-0985-z
dc.identifier.urihttps://hdl.handle.net/11454/46519
dc.identifier.volume13en_US
dc.identifier.wosWOS:000310468000005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherKorean Fiber Socen_US
dc.relation.ispartofFibers and Polymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzymatic modificationen_US
dc.subjectSynthetic fabricsen_US
dc.subjectThrombinen_US
dc.subjectImmobilizationen_US
dc.subjectBleedingen_US
dc.titleThrombin immobilization to enzymatic modified PET and PAN fabrics and their applicationsen_US
dc.typeArticleen_US

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