An In house ELISA using recombinant GRA1 and BAG1 proteins as antigen successfully detected Toxoplasma gondii infection in a flock of sheep suffering from abortions

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dc.authorscopusid57201433479
dc.authorscopusid23003351400
dc.authorscopusid56495590200
dc.authorscopusid23767084500
dc.authorscopusid57212213434
dc.authorscopusid23766969700
dc.authorscopusid24779770200
dc.contributor.authorKandemir, Ç.
dc.contributor.authorCan, H.
dc.contributor.authorKarakavuk, M.
dc.contributor.authorDöşkaya, M.
dc.contributor.authorErkunt, Alak, S.
dc.contributor.authorGürüz, A.Y.
dc.contributor.authorÜn, C.
dc.date.accessioned2024-08-25T18:32:06Z
dc.date.available2024-08-25T18:32:06Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractToxoplasma gondii is one of the main pathogens causing abortion in sheep. In this study, an in house ELISA using recombinant GRA1 and BAG1 proteins as antigen was used to detect T. gondii infection in a flock of sheep suffering from abortions. Serum samples collected from sheep (n = 23) the day after abortion were initially analyzed with a commercial ELISA using recombinant P30 (SAG1) protein as antigen and then by the in house ELISA using recombinant GRA1 and BAG1 proteins as antigen. Commercial ELISA detected anti-T. gondii IgG antibodies in 20 samples whereas in house ELISA detected anti-T. gondii IgG antibodies in 19 samples. The results of the remaining three serum samples that were seronegative by commercial ELISA were also seronegative by in house ELISA. According to the results of commercial ELISA and in house ELISA, the seroprevalence of ovine toxoplasmosis was 86.9% and 82.6%, respectively. When we accept the validated commercial ELISA as a reference method, the sensitivity and specificity of in house ELISA can be considered as 95% and 100%. These preliminary results show that in house ELISA using recombinant GRA1 and BAG1 proteins as antigen can detect ovine toxoplasmosis successfully. © 2023en_US
dc.identifier.doi10.1016/j.rvsc.2023.105054
dc.identifier.issn0034-5288
dc.identifier.scopus2-s2.0-85175043496en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.rvsc.2023.105054
dc.identifier.urihttps://hdl.handle.net/11454/100117
dc.identifier.volume165en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofResearch in Veterinary Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectELISAen_US
dc.subjectOvine toxoplasmosisen_US
dc.subjectRecombinant antigenen_US
dc.subjectSheepen_US
dc.titleAn In house ELISA using recombinant GRA1 and BAG1 proteins as antigen successfully detected Toxoplasma gondii infection in a flock of sheep suffering from abortionsen_US
dc.typeArticleen_US

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