Modification of Escherichia coli single-stranded DNA binding protein with gold nanoparticles for electrochemical detection of DNA hybridization

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dc.contributor.authorKerman, K
dc.contributor.authorMorita, Y
dc.contributor.authorTakamura, Y
dc.contributor.authorOzsoz, M
dc.contributor.authorTamiya, E
dc.date.accessioned2019-10-27T19:03:44Z
dc.date.available2019-10-27T19:03:44Z
dc.date.issued2004
dc.departmentEge Üniversitesien_US
dc.description.abstractThe unique binding event between Escherichia coli single-stranded DNA binding protein (SSB) and single-stranded oligonucleotides conjugated to gold (Au) nanoparticles is utilized for the electrochemical detection of DNA hybridization. SSB was attached onto a self-assembled monolayer (SAM) of single-stranded oligonucleotide modified Au nanoparticle, and the resulting Au-tagged SSB was used as the hybridization label. Changes in the Au oxidation signal was monitored upon binding of Au tagged SSB to probe and hybrid on the electrode surface. The amplified oxidation signal of Au nanoparticles provided a detection limit of 2.17 pM target DNA, which can be applied to genetic diagnosis applications. This work presented here has important implications with regard to combining a biological binding event between a protein and DNA with a solid transducer and metal nanoparticles. (C) 2004 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.aca.2003.12.067
dc.identifier.endpage174en_US
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.issn0003-2670en_US
dc.identifier.issn1873-4324en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage169en_US
dc.identifier.urihttps://doi.org/10.1016/j.aca.2003.12.067
dc.identifier.urihttps://hdl.handle.net/11454/38145
dc.identifier.volume510en_US
dc.identifier.wosWOS:000220818100006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNA biosensoren_US
dc.subjecthybridizationen_US
dc.subjectsingle-stranded DNA binding proteinen_US
dc.subjectgold nanoparticlesen_US
dc.subjectsquare wave voltammetryen_US
dc.titleModification of Escherichia coli single-stranded DNA binding protein with gold nanoparticles for electrochemical detection of DNA hybridizationen_US
dc.typeArticleen_US

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