Electrochemical glucose biosensing by pyranose oxidase immobilized in gold nanoparticle-polyaniline/AgCl/gelatin nanocomposite matrix

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dc.contributor.authorOzdemir C.
dc.contributor.authorYeni F.
dc.contributor.authorOdaci D.
dc.contributor.authorTimur S.
dc.date.accessioned2019-10-26T22:40:07Z
dc.date.available2019-10-26T22:40:07Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractA novel pyranose oxidase (PyOx) biosensor based on gold nanoparticles (AuNPs)-polyaniline(PANI)/AgCl/gelatin nanocomposite has been developed for the glucose detection. PyOx was immobilized on the surface of glassy carbon electrode (GCE) via the nanocomposite matrix. The electrode surface was imaged by scanning electron microscopy (SEM). Amperometric detection of the consumed oxygen during the enzymatic reaction was monitored at -0.7 V. After optimization studies, analytical characterization of the biosensor was carried out. The linear response of the AuNPs-AgCl/PANI/gelatin modified PyOx biosensor is found to be from 0.05 to 0.75 mM glucose with the equation of y = 2.043x + 0.253; R2 = 0.993. Finally, proposed biosensor was used to analyze glucose content in real samples. Obtained data from the biosensing system was compared with a commercial enzyme assay kit based on spectrophotometric Trinder reaction as a reference method. © 2009 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.foodchem.2009.05.087
dc.identifier.endpage385en_US
dc.identifier.issn0308-8146
dc.identifier.issn0308-8146en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage380en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2009.05.087
dc.identifier.urihttps://hdl.handle.net/11454/19989
dc.identifier.volume119en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofFood Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBionanocompositeen_US
dc.subjectGold nanoparticle (AuNP)en_US
dc.subjectPolyaniline (PANI)en_US
dc.subjectPyranose oxidase (PyOx)en_US
dc.titleElectrochemical glucose biosensing by pyranose oxidase immobilized in gold nanoparticle-polyaniline/AgCl/gelatin nanocomposite matrixen_US
dc.typeArticleen_US

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