Synthesis of albumin nanoparticles in a water-miscible ionic liquid system, and their applications for chlorambucil delivery to cancer cells

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dc.authoridCakan-Akdogan, Gulcin/0000-0002-6356-5979
dc.authoridKARAKOYUN, Cigdem/0000-0003-1150-7061
dc.authorscopusid13805842900
dc.authorscopusid57933216200
dc.authorscopusid57219241283
dc.authorscopusid57221300506
dc.authorscopusid57203016888
dc.authorscopusid24922534100
dc.authorwosidKARAKOYUN, Çiğdem/GZG-3522-2022
dc.authorwosidCakan-Akdogan, Gulcin/GRO-6030-2022
dc.contributor.authorAkdogan, Yasar
dc.contributor.authorSozer, Sumeyra Cigdem
dc.contributor.authorAkyol, Cansu
dc.contributor.authorBasol, Merve
dc.contributor.authorKarakoyun, Cigdem
dc.contributor.authorCakan-Akdogan, Gulcin
dc.date.accessioned2023-01-12T19:55:46Z
dc.date.available2023-01-12T19:55:46Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractSerum albumin has been a preferred protein to generate biodegradable and non-toxic nanoparticles (NPs) for drug delivery applications. Different methods applied for the preparation of serum albumin NPs mostly used organic solvents. Here, we prepared serum albumin NPs in an ionic liquid (IL) system. ILs are considered to be green and designer solvents with unique properties that can replace organic solvents in the synthesis of albumin NPs. Bovine serum albumin (BSA) proteins dissolved in water were trans-formed into BSA NPs in a water/ TritonTMX (TX-100), 1-butanol/1-butyl-3-methylimidazolium trifluo-romethanesulfonate (BmimCF3SO3) microemulsion-like system by using a high-speed homogenizer and crosslinker glutaraldehyde. The obtained BSA NPs have been used in drug loading and release studies with a hydrophobic anticancer drug chlorambucil (Chl). Drug loading increased as increasing the ratio of Chl incubated with BSA NPs. Monitoring the drug release by UV-Vis spectroscopy revealed a burst release at first 4 h, but two-thirds of drugs stayed with NPs upon diffusion method. On the other hand, cellular uptake of Chl loaded BSA NPs caused a significant MCF7 breast cancer cell death, whereas free Chl and unloaded BSA NPs did not have a significant effect on the cell viability. Furthermore, in vivo toxicity assessment of BSA NPs obtained in the IL system was conducted in the zebrafish animal model. It showed that zebrafish body is able to eliminate BSA NPs without any toxic side effects and encapsulation of Chl into NPs reduced the toxicity of free Chl. In summary, we showed that BSA NPs with size smaller than 200 nm could be prepared in BmimCF3SO3 mediated system. They can be used for Chl loading (up to 6.9 wt%) with a sustainable release and they induce significant cell death in Chl sensitive cancer cells up to 45% in 24 h. These results indicate that BSA NPs could be prepared alternatively in IL systems and used in drug delivery studies.(c) 2022 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTurkish Scientific and Technological Research Council (Tubitak) [118Z341]; IZTECH Center for Materials Research, IZTECH Biotechnology and Bioengineering Research Cen-teren_US
dc.description.sponsorshipThis work was financially supported by Turkish Scientific and Technological Research Council (Tubitak) via 1001 Program under grant 118Z341. Authors thank IZTECH Center for Materials Research, IZTECH Biotechnology and Bioengineering Research Cen-ter, IBG Optic Imaging Core Facility, IBG Zebrafish Facility staff for technical support, S. Senturk and H. al Otaibi for sharing cells, and Prof. Dr. M. M. Demir for DLS measurements.en_US
dc.identifier.doi10.1016/j.molliq.2022.120575
dc.identifier.issn0167-7322
dc.identifier.issn1873-3166
dc.identifier.issn0167-7322en_US
dc.identifier.issn1873-3166en_US
dc.identifier.scopus2-s2.0-85140135776en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.molliq.2022.120575
dc.identifier.urihttps://hdl.handle.net/11454/76748
dc.identifier.volume367en_US
dc.identifier.wosWOS:000884397300008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Molecular Liquidsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIonic liquidsen_US
dc.subjectAlbumin nanoparticleen_US
dc.subjectChlorambucilen_US
dc.subjectDrug loadingen_US
dc.subjectCell viabilityen_US
dc.subjectDrug-Deliveryen_US
dc.subjectStarch Nanoparticlesen_US
dc.subjectMicroemulsion Systemen_US
dc.subjectControlled-Releaseen_US
dc.subjectBsa Nanoparticlesen_US
dc.subjectBound Paclitaxelen_US
dc.subjectSurfaceen_US
dc.subjectToolen_US
dc.titleSynthesis of albumin nanoparticles in a water-miscible ionic liquid system, and their applications for chlorambucil delivery to cancer cellsen_US
dc.typeArticleen_US

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