Apoptosis-inducing activity of safflower (Carthamus tinctorius L.) seed oil in lung, colorectal and cervix cancer cells

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dc.contributor.authorGuner, Adem
dc.contributor.authorKizilsahin, Sadi
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorYavasoglu, Nefise Ulku Karabay
dc.date.accessioned2020-12-01T12:02:25Z
dc.date.available2020-12-01T12:02:25Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractCarthamus tinctorius L. (Safflower) has been often preferred because of rich fatty acid, flavonoid, alkaloid, and polysaccharide contents in its different parts in medicine and industrial area. Although its antioxidant, antienflamatuar, and antitumor properties have been proven in many studies, the mechanism underlying the anticancer activity is still more unclear. This study was first conducted to elucidate the apoptotic gene expression changes in human colorectal (CaCo-2), lung (A549), and cervix cancer (HeLa) cells after exposure to safflower seed oil (SFO). Cytotoxic activity of cancer cells was evaluated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl2H-tetrazoliumbromide) assay and then, total RNA derived from cell lines to analyze the gene expression profile on Real-Time Ready Human Apoptosis Panel 96 was used. MTT results showed that SFO greatly inhibited A549, CaCo-2 and HeLa cell proliferation, with a value of IC50 of 1.26, 3.92 and 13.12 mu g/ml, respectively. According to the cDNA microarray analysis, 56 genes were interpreted in connection with extrinsic, intrinsic, PI3K/AKT, JAK/STAT, and NF kappa B pathways. SFO treatments triggered apoptosis through the caspase-dependently pathway along with upregulated the expressions of many pro-apoptotic genes in the extrinsic and intrinsic pathway in HeLa cells. However, in A549 and CaCo-2 cells, SFO treatments were inhibited cell survival mechanism through frequently caspase-independent genes following downregulated the expression of anti-apoptotic genes. It is noteworthy that although cancer cells have different sensitivity, SFO induced apoptosis through different pathways. Taken together, SFO, as a natural resource, has the potential to be used as a promising agent against cancer, especially in gene therapy level.en_US
dc.description.sponsorshipEge University BAP projectEge University [2013/FEN/051]en_US
dc.description.sponsorshipThe study is supported by Ege University BAP project (2013/FEN/051).en_US
dc.identifier.doi10.2478/s11756-020-00458-2
dc.identifier.endpage1471en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issn0006-3088en_US
dc.identifier.issn1336-9563en_US
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-85089806374en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1465en_US
dc.identifier.urihttps://doi.org/10.2478/s11756-020-00458-2
dc.identifier.urihttps://hdl.handle.net/11454/62676
dc.identifier.volume75en_US
dc.identifier.wosWOS:000516463300002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnticanceren_US
dc.subjectApoptosisen_US
dc.subjectCancer cellen_US
dc.subjectCytotoxicityen_US
dc.subjectSaffloweren_US
dc.titleApoptosis-inducing activity of safflower (Carthamus tinctorius L.) seed oil in lung, colorectal and cervix cancer cellsen_US
dc.typeArticleen_US

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