Optimization of the production of beta-carotene from molasses by Blakeslea trispora: a statistical approach

Basit Öğe Kaydı
dc.contributor.authorGoksungur, Y
dc.contributor.authorMantzouridou, F
dc.contributor.authorRoukas, T
dc.date.accessioned2019-10-27T18:44:12Z
dc.date.available2019-10-27T18:44:12Z
dc.date.issued2002
dc.departmentEge Üniversitesien_US
dc.description.abstractThe effect of pretreatment of molasses, nitrogen sources, natural oils, fatty acids, antioxidant, precursors, and mixtures of the above substances on beta-carotene production by Blakeslea trispora in shake flask culture was investigated. Also, a central composite design was employed to determine the maximum beta-carotene concentration at optimum values for the process variables (linoleic acid, kerosene, antioxidant). The highest concentration of the carotenoid pigment was obtained in molasses solution treated with invertase. Corn steep liquor and yeast extract at concentrations of 5.0% and 0.5% (w/v), respectively, increased slightly the concentration of beta-carotene, while the natural oils, fatty acids, and precursors (except kerosene) did not improve the production of pigment when they were added separately to the medium. On the other hand, the mixture of linoleic acid, kerosene and antioxidant increased significantly the concentration of beta-carotene. The fit of the model was found to be good. Linoleic acid, kerosene and antioxidant had a strong linear effect on beta-carotene concentration. The concentration of beta-carotene was significantly affected by linoleic acid-antioxidant and kerosene-antioxidant interactions as well as by the negative quadratic effects of these variables. The interaction between linoleic acid-kerosene had no significant linear effect. Maximum P-carotene concentration (790.0 mg dm(-3)) was obtained in culture grown in molasses solution supplemented with linoleic acid (30.74 g dm(-3)), kerosene (27.79 g dm(-3)) and antioxidant (10.22 g dm(-3)). (C) 2002 Society of Chemical Industry.en_US
dc.identifier.doi10.1002/jctb.662
dc.identifier.endpage943en_US
dc.identifier.issn0268-2575
dc.identifier.issn1097-4660
dc.identifier.issn0268-2575en_US
dc.identifier.issn1097-4660en_US
dc.identifier.issue8en_US
dc.identifier.startpage933en_US
dc.identifier.urihttps://doi.org/10.1002/jctb.662
dc.identifier.urihttps://hdl.handle.net/11454/37282
dc.identifier.volume77en_US
dc.identifier.wosWOS:000177228900011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Chemical Technology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeet molassesen_US
dc.subjectBlakeslea trisporaen_US
dc.subjectbeta-caroteneen_US
dc.subjectresponse surface methodologyen_US
dc.titleOptimization of the production of beta-carotene from molasses by Blakeslea trispora: a statistical approachen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu