In vitro evaluation of apoptosis detection by Tc-99m-tetrofosmin in MCF-7 breast cancer cell line

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dc.contributor.authorTabar, E. Bayrak
dc.contributor.authorLambrecht, F. Yurt
dc.contributor.authorGunduz, C.
dc.contributor.authorYucebas, M.
dc.date.accessioned2019-10-27T21:24:14Z
dc.date.available2019-10-27T21:24:14Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractRadiolabeled molecules have an important role to evaluate tumor characteristics such as aggressiveness, and to identify the effectiveness of cancer treatments such as chemotherapy and radiotherapy. Various radionuclide (F-18, Tc-99m, I-124) labeled molecules can be used apoptosis detection by estimating decrescendos cell viability after therapy. Tc-99m-tetrofosmin which is used as a myocardial perfusion imaging agent in routine and at the same time is known to accumulate in various tumors including breast tumor. The aim of this study was to assess the utility of Tc-99m-tetrofosmin for monitoring the early response of MCF-7 breast cancer to chemotherapy. To evaluate the role of Tc-99m-tetrofosmin in vitro chemotherapy, the uptake ratio was determined using MCF-7 breast cancer line after the cells had been treated with cisplatin. When we examined the apoptotic ratios which induced with different dose of cisplatin in MCF-7 breast cancer cells by using Annexin V and TUNEL methods, it was observed that the rate of apoptosis increased with soaring dose. The uptake rates of Tc-99m-tetrofosmin in MCF-7 cell line in the chemotherapeutic groups were lower than it is in the control group (p < 0.01). The negative correlation between uptake ratios and apoptotic rates shows that Tc-99m-tetrofosmin may be used a radiopharmaceutical for evaluating chemotherapy response. Tc-99m-tetrofosmin might be probably useful as an imaging agent for estimation of early chemotherapy response in breast cancer.en_US
dc.description.sponsorshipDepartment of Scientific Projects at Ege University, Izmir, TurkeyEge Universityen_US
dc.description.sponsorshipThis research was supported from Department of Scientific Projects at Ege University, Izmir, Turkey. We would like to thank Nukleer Urunler AS because of gifted tetrofosmin.en_US
dc.identifier.doi10.1007/s10967-011-1005-2
dc.identifier.endpage844en_US
dc.identifier.issn0236-5731
dc.identifier.issn0236-5731en_US
dc.identifier.issue3en_US
dc.identifier.startpage839en_US
dc.identifier.urihttps://doi.org/10.1007/s10967-011-1005-2
dc.identifier.urihttps://hdl.handle.net/11454/44446
dc.identifier.volume288en_US
dc.identifier.wosWOS:000290040000028en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Radioanalytical and Nuclear Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMCF-7en_US
dc.subjectBreast canceren_US
dc.subjectApoptosisen_US
dc.subjectTc-99m-tetrofosminen_US
dc.subjectCisplatinen_US
dc.titleIn vitro evaluation of apoptosis detection by Tc-99m-tetrofosmin in MCF-7 breast cancer cell lineen_US
dc.typeArticleen_US

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