A New Tc-99m Labeled Peptide: Tc-99m beta-Casomorphin 6, Biodistribution and Imaging Studies on Rats

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dc.contributor.authorErtay, Turkan
dc.contributor.authorUnak, Perihan
dc.contributor.authorEren, Mine Sencan
dc.contributor.authorBiber Muftuler, Fazilet Zumrut
dc.contributor.authorOzdogan, Ozhan
dc.contributor.authorUlker, Ozden
dc.contributor.authorYesilagac, Reyhan
dc.contributor.authorDurak, Hatice
dc.date.accessioned2019-10-27T11:23:15Z
dc.date.available2019-10-27T11:23:15Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractPeptide radiopharmaceuticals have an increasing significance in nuclear medicine practice. beta-casomorphin is a digestive peptide with 6 amino acids (Tyr-Pro-Phe-Pro-Gly-Pro). N terminal amino acid chain mainly tyr-pro-phe-pro structured exogen opioid peptid type beta cosomorphin are beta-receptor agonistic activity with priority. Animal studies show that beta-casomorphins can act as opioid receptor agonists. The aim of this study was to label beta-casomorphin with (99m) Tc and to examine its usefulness as an opioid receptor binding radiopharmaceutical in Albino Wistar rats and cancer cells. beta-casomorphin was labeled with (99m) Tc radionuclide using bifunctional chelating agent. Quality control studies were done by Instant Thin layer chromatography (ITLC) and High performance liquid chromatography (HPLC) methods. Binding efficiency of the compound was more than 99%. It was observed as stable for at least 3 h at room temperature. Lipophilicity was also performed for labeled molecule. Imaging studies for (99m) Tc labeled molecule was done in rats by using gamma camera. For biodistribution studies; (99m) Tc labeled molecule was injected to the rats from tail vein and radioactivity per gr weight of each organ was measured as count per second (cps). Receptor specificity was evaluated in imaging and biodistribution studies in experimental animals. Cell labeling studies were also performed on breast and ovarien cancer cells. In terms of evaluating the biodistribution of (99m) Tc-beta-casomorphin molecule in rats, uterus and ovary displayed high involvement. It was also confirmed by cell labeling studies. If the radiopharmaceutical is radiolabeled with therapeutic radionuclides it would be useful for therapy for uterus, ovary and breast tumors.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG-104T241]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK), the author wish to thank TUBITAK for financial support (Grant No: TBAG-104T241).en_US
dc.identifier.doi10.9775/kvfd.2016.15691
dc.identifier.endpage22en_US
dc.identifier.issn1300-6045
dc.identifier.issn1300-6045en_US
dc.identifier.issue1en_US
dc.identifier.startpage15en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2016.15691
dc.identifier.urihttps://hdl.handle.net/11454/33153
dc.identifier.volume23en_US
dc.identifier.wosWOS:000388115200003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbeta-casomorphinen_US
dc.subjectCancer cellen_US
dc.subjectPeptideen_US
dc.subjectTc-99men_US
dc.subjectreceptoren_US
dc.titleA New Tc-99m Labeled Peptide: Tc-99m beta-Casomorphin 6, Biodistribution and Imaging Studies on Ratsen_US
dc.typeArticleen_US

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