Critical time point for apoptotic cell death in an experimental ischemia/reperfusion model and the effect of N-acetylcystein

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dc.contributor.authorOzsarlak-Sozer, Gonen
dc.contributor.authorEmre, Mustafa
dc.contributor.authorDemirkol, Serhat
dc.contributor.authorAcikalin, Arbil
dc.contributor.authorCetiner, Salih
dc.contributor.authorTopcu, Zeki
dc.contributor.authorDemir-Dora, Devrim
dc.date.accessioned2019-10-27T11:07:39Z
dc.date.available2019-10-27T11:07:39Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: Kidney transplantation is an important treatment option in end stage renal failure. Tissue death may be an important problem when a kidney is removed from a cadaver and transported to a donor a long distance away. The purpose of this study is to determine the critical time point for apoptotic cell death in a renal ischemia/reperfusion model and determine the effects of N-acetylcystein on apoptosis induced by ischemia injury. Methods: Apoptotic cell death after induced renal ischemia followed by reperfusion, was estimated in a group of Wistar albino rats by immunoflourescence and ELISA techniques. N-acetylcystein, an antioxidant agent, was given to the rats to study the effect on apoptosis. Tissues were examined immunohistochemically at 0, 1 h, 24 h, 5 days and 10 days for detection of apoptotic cells. Results: Our results showed that an ischemia for 60 min followed by reperfusion for 60 min triggered apoptosis. Moreover, N-acetylcystein significantly diminished both the ischemia/reperfusion damage and apoptosis. Conclusion: We anticipate our results would be important for kidney transplantation in estimating the critical time point for apoptosis and administration of N-acetylcystein prior to removal of the organ may be important in delaying the onset of apoptosis.en_US
dc.identifier.doi10.1515/tjb-2016-0306
dc.identifier.endpage272en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issn0250-4685en_US
dc.identifier.issn1303-829Xen_US
dc.identifier.issue3en_US
dc.identifier.startpage265en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2016-0306
dc.identifier.urihttps://hdl.handle.net/11454/32060
dc.identifier.volume42en_US
dc.identifier.wosWOS:000405121500004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectKidneyen_US
dc.subjectTransplantationen_US
dc.subjectIschemia/reperfusionen_US
dc.subjectApoptosisen_US
dc.subjectN-acetylcysteinen_US
dc.titleCritical time point for apoptotic cell death in an experimental ischemia/reperfusion model and the effect of N-acetylcysteinen_US
dc.typeArticleen_US

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